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  6. Thermo Scientific™ HisPur™ Ni-NTA Resin

Thermo Scientific™ HisPur™ Ni-NTA Resin

Artikelnummer. 10459164 Shop alle Thermo Scientific produkter
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Quantity:
10 mL
100 mL
500 mL
Pakningsstørrelse:
100mL
10mL
500mL
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Artikelnummer. 10459164 Leverandør Thermo Scientific™ Leverandørnr. 88223

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Beskrivelse

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Perform routine affinity purification of His-tagged fusion proteins with Thermo Scientific™ HisPur Ni-NTA Resin, a high-capacity, high-performance nickel-IMAC resin.

The specially prepared nickel-NTA support consists of beaded agarose derivatized with the nitrilotriacetic acid (NTA) chelation moiety and loaded with divalent nickel ions (Ni2+). The immobilized metal affinity chromatography (IMAC) resin provides exceptional binding capacity and performance for recombinant His-tagged protein purification.

HisPur Ni-NTA Resin is available in several package sizes of resin slurry, three sizes of centrifuge-ready columns, complete purification kits, two sizes of FPLC-ready chromatography cartridges, and 96-well filter plates for high through-put needs. With these options, high-yield His-tag protein purification is possible at nearly any scale.

Highlights:

  • High capacity – bind up to 60mg of 6xHis-tagged protein per milliliter of resin
  • Versatile – purify proteins using native or denaturing conditions
  • Compatible – use with Thermo Scientific Cell Lysis Reagents and a variety of buffer additives
  • Flexible – available in multiple formats including bulk resin, spin columns and chromatography cartridges
  • Cost-effective – reuse the same batch of resin at least five times
  • Easy to use – pre-formulated buffers available for kit formats

Available Formats:

  • Resin Slurries – crosslinked 6% beaded agarose; 10mL, 100mL, 500mL bottles
  • Spin Columns – 0.2mL (microcentrifuge), 1mL, 3mL columns
  • Purification Kits – complete kits in all three column sizes
  • Chromatography Cartridges – compatible with automated liquid chromatography systems and with syringe processing
  • Bulk and Custom – let us know your needs for bulk HisPur Ni-NTA Resin

Includes:

Kits contain HisPur Ni-NTA Columns (store at 4°C)

TRUSTED_SUSTAINABILITY

Tekniske data

Capacity (Metric) Up to 60 mg of 6xHis-Tagged Protein Binding per mL Resin
Description HisPur Ni-NTA Resin
Content And Storage Store at 4°C
Form Liquid Suspension
Product Line HisPur
Quantity 500 mL
Target His-Tagged Protein
Type Resin
Stationary Phase Divalent Nickel, Metal Chelate
Format Bottle
Column Type Affinity, Agarose Resin
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For Research Use Only. Not for use in diagnostic procedures.

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