H-2D[d] Mouse anti-Mouse, FITC, Clone: 34-2-12, BD

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
15851549	BD Biosciences 562001	50 μg
15887288	BD Biosciences 553579	0.5 mg

Description

The 34-2-12 antibody (also known as 34-2-12S) recognizes the α3 domain of the H-2D[d]. The binding of the antibody to its epitope is independent of the α1 and α2 domains and β2 microglobulin. It cross-reacts with cells of the C3H.LG/Ckc strain. Reactivity with other haplotypes (eg, b, f, k, p, q, r, s) has not been observed. Soluble mAb 34-2-12 blocks binding of the Ly-49A-expressing T lymphoma EL4 to immobilized H-2D[d]. However, further studies utilizing this mAb indicate that the α3 domain is not involved in the interaction between Ly-49A, or Ly-49G2, and H-2D[d].

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Specifications

• Antigen: H-2D[d]
• Concentration: 0.5mg/mL
• Classification: Monoclonal
• Host Species: Mouse
• Target Species: Mouse
• Immunogen: (C57BL/6 x DBA/2)F1 mouse splenocytes
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Clone: 34-2-12
• Applications: Flow Cytometry
• Conjugate: FITC
• Regulatory Status: RUO
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Isotype: IgG2a κ
• Purification Method: Affinity Purified

For Research Use Only.