Thermo Scientific™ GlycoLink™ Immobilization Kits and Resin

Immobilize polyclonal antibodies or other glycoproteins, via periodate-oxidized sugars, onto hydrazide-activated polyacrylamide beads for AP, IP or pull-down assays.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 88942

GLYCOLINK MICRO IMMOBILIZATION KITSegregation: Separated from ammonium compounds, cy

UNSPSC: 41106510

Code: PN

Additional Details:
Additional Details: CAS Number: 232-197-6 Weight: 0.01000kg Transport: UN number : 3087 Chem class : 1 Pack group : II



Product Code. 11638843

Quantity Price
1 £ 294.0 / 1 set
Estimated Shipment date
from Supplier 08-02-2017
Add to basket


Description and Specification

Specification

Formulation Multi-component kit with 10 x 0.1mL UltraLink Hydrazide Resin
Kit Contents Aniline, 0.2mL
Sufficient For Immobilizing 50 to 500ug of glycoprotein per column
Description GlycoLink Micro Immobilization Kit
Quantity 10 column kit
Support Type Acrylamide

The GlycoLink Kits use hydrazide-activated resin and a special catalyst to prepare affinity columns with polyclonal antibodies, which typically have abundant carbohydrates (glycosylation) on their Fc portions. Any glycosylated proteins (even monoclonal antibodies) that contain periodate-oxidizable carbohydrate groups can be immobilized with these kits to make reusable affinity columns. Antibodies immobilized by this hydrazide immobilization chemistry have unobstructed antigen-binding sites and optimal purification capability. Prepared columns with stable glycoproteins or antibodies can be regenerated and reused at least five times for affinity-purification without significant loss in binding capacity.

Highlights:

  • High-capacity – immobilize 1 to 10mg of oxidized antibody or other glycoprotein per milliliter of resin

  • Fast and efficient – immobilize in as few as 30 minutes; achieve at least 90% coupling of most glycoproteins in less than 4 hours (efficiency depends on the amount and type of glycosylation)

  • Specific immobilization – the hydrazide-activated UltraLink Resin conjugates only to purified glycoproteins whose sugar (e.g., sialic acid) groups have been gently oxidized with periodate

  • Stable attachment – resonance structure of the hydrazone bonds are sufficiently stable for multiple affinity purifications with one batch of prepared resin; no stabilization step is required

  • Preserves antibody function – immobilizes IgG via the Fc region, thereby keeping both antigen binding sites available for capturing target

  • Convenient sizes – choose from two complete 10-column kits: one with 1mL columns and one with 0.1mL columns

UltraLink Hydrazide Resin:

  • Ligand: hydrazide
  • Support: Thermo Scientific UltraLink Biosupport
  • Spacer arm: 22 atoms
  • Loading: activated with at least 15μmol hydrazide per milliliter of settled resin
  • Format: 50% resin slurry in 0.02% sodium azide

Includes:

Kits contain GlycoLink Columns; coupling buffer, coupling catalyst and wash buffer; oxidizing reagent; desalting columns, PBS buffer pack; and column accessories

Recommended for:

Purify antigens using covalently coupled antibodies; Determine binding partners for a glycoprotein of interest; Perform large-scale affinity purifications