Invitrogen™ GeneBLAzer™ TRβ-UAS-bla HEK 293T Cells

GeneBLAzer™ TRβ-UAS-bla HEK 293T Cells

Brand:  Invitrogen™ K1686

Product Code. 10686214

Description

TR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Thyroid hormone receptor beta(TR beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UASbla HEK 293T cell line. GeneBLAzer™UAS-bla HEK 293T cells stably express a betalactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-TR beta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. TR beta-UAS-bla HEK 293T cells are functionally validated for Z' and EC₅₀ concentrations of T3 Thryoid hormone. In addition, TR beta-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

Specifications

• Content And Storage: -80°C/Liquid Nitrogen
• Detection Method: Fluorescent
• Includes: TR beta-UAS-bla HEK 293T cells (K1248)
• Sample Type: 293T (HEK)

• Description: GeneBLAzer™ TRβ-UAS-bla HEK 293T Cells, 2 x 106cells, ∽2,000,000 cells/vial at 2,000,000 cells/mL Quantity of Cells, Fluorescent Detection Method, Nuclear receptors Druggable Target, BLA (Beta-Lactamase) Reporter, Adherent Growth Properties, –80°C/Liquid Nitrogen Storage
• For Use With (Application): Pharma and biopharma, drug discovery and development, target and lead identification and validation, G-protein coupled receptor biology, cell-based GPCR reporter assays
• Quantity: 2 x 106 Cells
• Type: Cell Line

For Research Use Only. Not for use in diagnostic procedures.