missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Cell Analysis Products
  4. Cell Based Assays
  5. General Cell Based Assays
  6. 10368763

Thermo Scientific™ GeneBLAzer™ LXRβ-UAS-bla HEK 293T Cells

Product Code. 10368763
Click to view available options
Quantity:
2 x 106 Cells
Unit Size:
Each
This item is not returnable. View return policy

Product Code. 10368763

Brand: Thermo Scientific™ K1699

Please to purchase this item. Need a web account? Register with us today!

This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
 View alternative products

This item is not returnable. View return policy

Description

LXR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Liver-X receptor-beta (LXR beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line.

LXR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Liver-X receptor-beta (LXR beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-LXR beta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. LXR beta-UAS-bla HEK 293T 293 cells are functionally validated for Z' and EC50 concentrations of TO901317. In addition, cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

TRUSTED_SUSTAINABILITY

Specifications

Alternate Agonists DHT
Antagonists Cyp Acetate, Spirolactone, OHF
Assay Entry Cell-based beta lactamase reporter gene
Cell State Dividing Cells
Content And Storage LXR beta-UAS-bla HEK 293T cells (K1131)

Shipping: Dry Ice
Storage: -80°C (liquid nitrogen), Liquid Nitrogen
Detection Method Fluorescence, Fluorescent
For Use With (Application) Nuclear Receptors
Species Human
Druggable Target Nuclear Receptors
No. of Cells 2 x 106 Cells
Primary Agonist R1881
Product Line GeneBLAzer
Quantity 2 x 106 Cells
Product Type HEK 293T Cells
Reporter Gene BLA (Beta-Lactamase)
Target Entry LXR beta, NR1H2, LXRB
Type Dividing Cells
Show More Show Less

For Research Use Only. Not for use in diagnostic procedures.

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.