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Thermo Scientific™ GeneBLAzer™ GRPR CHO-K1 DA Assay Kit

Product Code. 10676214
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Quantity:
8 x 106 Cells
Unit Size:
Each
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Product Code. 10676214

Brand: Thermo Scientific™ K1637

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Description

The GeneBLAzer™ GRPR-NFAT-bla CHO-K1 cells contain the human Gastrin Releasing Peptide Receptor (GRPR) stably integrated into the GeneBLAzer™ NFAT-bla CHO-K1 cell line.

The GeneBLAzer™ GRPR-NFAT-bla CHO-K1 cells contain the human Gastrin Releasing Peptide Receptor (GRPR) stably integrated into the GeneBLAzer™ NFAT-bla CHO-K1 cell line. GeneBLAzer™ NFAT-bla CHO-K1 (Cat. No. K1447) contains a beta-lactamase (bla) reporter gene under control of a NFAT response element stably integrated into CHO-K1 cells.

The GRPR-NFAT-bla CHO-K1 cells are functionally validated for Z'-factor and EC50 concentrations using established ligands. In addition, GRPR-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary. Division Arrested cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.

TRUSTED_SUSTAINABILITY

Specifications

Accession Number NM_005314.2
Assay Entry Cell-based beta lactamase reporter gene
Cell State Division-Arrested Cells
Content And Storage Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).

Includes:
• GeneBLAzer™ GRPR CHO-K1 DA cells
• LiveBLAzer™-FRET B/G Loading Kit, 70 μg
• Solution D
• GeneBLAzer™ GRPR CHO-K1 DA Assay Protocol
• LiveBLAzer™-FRET B/G Loading Protocol
• Certificate of Analysis

The GeneBLAzer™ GRPR CHO-K1 DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Detection Method Fluorescence
For Use With (Application) GPCRs
Gene Symbol GRPR
Population Status Single Cell Clone Origin
Product Line GeneBLAzer, T-REx
Quantity 8 x 106 Cells
Reporter Gene BLA (Beta-Lactamase)
Target Entry GRPR
Type Division-Arrested Cells Assay Kit
Cell Line CHO-K1
System Type GeneBLAzer™
Druggable Target GPCR
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For Research Use Only. Not for use in diagnostic procedures.

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