Thermo Scientific™ GeneBLAzer™ GR-UAS-bla HEK 293T Cells

Product Code. 10083913

Brand: Thermo Scientific™ K1687

Description

GR-UAS-bla HEK 293T cells contain the ligandbinding domain (LBD) of the human Gluocorticoid receptor (GR) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line.

GR-UAS-bla HEK 293T cells contain the ligandbinding domain (LBD) of the human Gluocorticoid receptor (GR) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™UAS-blaHEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-GR (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. GR-UAS-bla HEK 293T cells are functionally validated for Z' and EC₅₀ concentrations of Dexamethasone. In addition, GR-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

Specifications

• Alternate Agonists: Tetrac, T4
• Antagonists: 1-850
• Assay Entry: Cell-based beta lactamase reporter gene
• Cell State: Dividing Cells
• Content And Storage: GR-UAS-bla HEK 293T cells (K1687)
  
  Shipping: Dry Ice
  Storage: -80°C (liquid nitrogen), Liquid Nitrogen
• Detection Method: Fluorescence, Fluorescent
• Description: GeneBLAzer™ GR-UAS-bla HEK 293T Cells, 2 x 106 Cells, ∽2,000,000 cells/vial at 2,000,000 cells/mL Quantity of Cells, 1-850 Antagonists, Fluorescent Detection Method, Nuclear Receptors Druggable Target, BLA (Beta-Lactamase) Reporter, Liquid Nitrogen Storage
• For Use With (Application): Pharma and biopharma, target and lead identification and validation
• Species: Human
• Druggable Target: Nuclear Receptors
• Includes: GeneBLAzer™ GR-UAS-bla HEK 293T cells - Includes: GR-UAS-bla HEK 293T cells, Protocol, Certificate of Analysis
• No. of Cells: 2 x 10⁶ Cells
• Primary Agonist: T3 Hormone
• Product Line: GeneBLAzer
• Quantity: 2 x 106 Cells, 2 x 10⁶ cells
• Product Type: HEK 293T Cells
• Sample Type: 293T (HEK)
• Reporter Gene: BLA (Beta-Lactamase)
• Target Entry: NR3C1, GR
• Type: Cell Line

For Research Use Only. Not for use in diagnostic procedures.