Invitrogen™ GeneBLAzer™ GR-UAS-bla HEK 293T Cells

GeneBLAzer™ GR-UAS-bla HEK 293T Cells

Brand:  Invitrogen™ K1687

Product Code. 10083913

Description

GR-UAS-bla HEK 293T cells contain the ligandbinding domain (LBD) of the human Gluocorticoid receptor (GR) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™UAS-blaHEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-GR (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. GR-UAS-bla HEK 293T cells are functionally validated for Z' and EC₅₀ concentrations of Dexamethasone. In addition, GR-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

Specifications

• Content And Storage: Liquid Nitrogen
• Detection Method: Fluorescent
• Includes: GeneBLAzer™ GR-UAS-bla HEK 293T cells - Includes: GR-UAS-bla HEK 293T cells, Protocol, Certificate of Analysis
• Sample Type: 293T (HEK)

• Description: GeneBLAzer™ GR-UAS-bla HEK 293T Cells, 2 x 106 Cells, ∽2,000,000 cells/vial at 2,000,000 cells/mL Quantity of Cells, 1-850 Antagonists, Fluorescent Detection Method, Nuclear Receptors Druggable Target, BLA (Beta-Lactamase) Reporter, Liquid Nitrogen Storage
• For Use With (Application): Pharma and biopharma, target and lead identification and validation
• Quantity: 2 x 106 Cells
• Type: Cell Line

For Research Use Only. Not for use in diagnostic procedures.