Invitrogen™ GeneBLAzer™ ERα-UAS-bla GripTite™ Cells

GeneBLAzer™ ERα-UAS-bla GripTite™ Cells

Brand:  Invitrogen™ K1688

Product Code. 10135793

Description

ER alpha-UAS-bla GripTite™cells contain the ligand-binding domain (LBD) of the human Estrogen receptor alpha (ER alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla GripTite™ cell line. GeneBLAzer™UAS-bla GripTite™ cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-ER alpha (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. ER alpha-UAS-bla GripTite™ cells are functionally validated for Z' and EC₅₀ concentrations of 17-beta Estradiol. In addition, ER alpha-UAS-bla GripTite™cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

Specifications

• Content And Storage: Liquid nitrogen, room temperature
• Detection Method: Fluorescent
• Includes: ER alpha-UAS-bla GriptTite™ cells
• Sample Type: GripTite™ Cells

• Description: GeneBLAzer™ ERα-UAS-bla GripTite™ Cells, 2 x 106 Cells, ∽2,000,000 cells/vial at 2,000,000 Cells/mL Quantity of Cells, Cortisone/Mifepristone/Progesterone Antagonists, Fluorescent Detection Method, Nuclear Receptors Druggable Target, BLA (Beta-Lactamase) Reporter, Liquid Nitrogen Storage
• For Use With (Application): Pharma and biopharma, drug discovery and development, target and lead identification and validation, G-protein coupled receptor biology, cell-based GPCR reporter assays
• Quantity: 2 x 10^6 Cells
• Type: Cell Line

For Research Use Only. Not for use in diagnostic procedures.