Invitrogen™ GeneBLAzer™ ERβ-UAS-bla GripTite™ Cells

GeneBLAzer™ ERβ-UAS-bla GripTite™ Cells

Brand:  Invitrogen™ K1689B

Product Code. 10145793

Description

ER beta-UAS-bla GripTite™ 293 cells contain the ligand-binding domain (LBD) of the human Estrogen receptor beta (ER beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla GripTite™ 293 cell line. GeneBLAzer™UAS-bla GripTite™ 293 cells stably express a betalactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-ER beta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. ER beta-UAS-bla GripTite™293 cells are functionally validated for Z' and EC₅₀ concentrations of 17-beta Estradiol. In addition, ER beta-UAS-bla GripTite™293 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time. Additional testing data using alternate stimuli are also available.

Specifications

• Content And Storage: Liquid nitrogen, room temperature
• Detection Method: Fluorescent
• Includes: GeneBLAzer™ ER beta -UAS-bla GripTite™ cells Includes: ER beta-UAS-bla GripTite™ cells, Protocol, Certificate of Analysis
• Sample Type: GripTite™ 293

• Description: GeneBLAzer™ ERβ-UAS-bla GripTite™ Cells, 2 x 106 Cells, Fluorescent Detection Method, Nuclear Receptors Druggable Target, BLA (Beta-Lactamase) Reporter, Liquid Nitrogen Storage
• For Use With (Application): Pharma and biopharma, drug discovery and development, target and lead identification and validation, G-protein coupled receptor biology, cell-based GPCR reporter assays
• Quantity: 2 x 106 Cells
• Type: Cell Line

For Research Use Only. Not for use in diagnostic procedures.