Thermo Scientific™ GeneBLAzer™ ER Beta DA Assay Kit

GeneBLAzer™ER beta DA(Division Arrested) cells and ER beta-UAS-bla GripTite™ 293 cells contain the ligand-binding domain (LBD) of the human Estrogen receptor beta (ER beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla GripTite™ 293 cell line.

Brand:  Thermo Scientific™ K1399

Product Code. 10665685

Description

GeneBLAzer™UAS-bla GripTite™ 293 cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-ER beta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both ER beta DA cells and ER beta-UAS-bla GripTite™ 293 cells are functionally validated for Z' and EC₅₀ concentrations of 17-beta Estradiol. In addition, ER beta-UAS-bla GripTite™ 293 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time. Additional testing data using alternate stimuli are also available.

Specifications

• Alternate Agonists: Cholic Acid, Deoxycholic Acid
• Assay Entry: Cell-based beta lactamase reporter gene
• Content And Storage: Shipping: Dry IceStorage: -80°C/Liquid NitrogenSufficient division-arrested cells and substrate to assay one 384-well plate. Includes:ER beta HEK 293 T DA cells (K1399A)LiveBLAzer™-FRET B/G Loading Kit, 70 μg
• Gene ID (Entrez): 2100
• Primary Agonist: Chenodeoxycholic Acid (CDCA)
• Reporter Gene: BLA (Beta-Lactamase)
• Cell Line: 293T (HEK)
• Druggable Target: Nuclear Receptors

• Antagonists: (z)-guggulsterone
• Cell State: Division-Arrested Cells
• Detection Method: Fluorescent
• Gene Symbol: ERβ
• Product Line: GeneBLAzer™
• Target Entry: ER beta, ESR2, ERB, NR3A2
• System Type: GeneBLAzer™

For Research Use Only. Not for use in diagnostic procedures.