GE Healthcare Ni Sepharose™ 6 Fast Flow

Combines the advantages of Ni2 for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform.   GE Healthcare Lifescience™ Ni Sepharose™ 6 Fast Flow is part of a range of IMAC chromatography media for capture and intermediate purification.

Overview
Brand: GE Healthcare

Manufacturer Part Number: 17-5318-06

5 ML NI SEPHAROSE(TM) 6 FAST FLOW 5ML STORE ATambient Store at Room Temperature

UNSPSC: 41116104

Code: GE

Additional Details:
Additional Details: Weight: 0.05400kg



Product Code. 11540814

Quantity Price
1 £ 55.71 / 5mL
Estimated Shipment
23-12-2016
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Description and Specification

Specification

Buffer 0.1M HCl, 0.1M NaOH, 8M Urea
Product Type Ni Sepharose 6 FF chromato graphy media
Quantity 5mL
Height (Metric) 250mm
Flow Rate 600cm/hr.
Max. Pressure 1 bar
pH Range 3 to 12
Particle Size 90μm
Support Type 6% Cross-linked Agarose
Storage Requirements 4° to 30°C

Ni Sepharose 6 Fast Flow is a member of the GE Healthcare range of IMAC chromatography media for capture and intermediate purification.

  • Designed for scaling up purification of histidine-tagged proteins.
  • High protein binding capacity and minimal leakage of Ni2+ ions.
  • As a BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
  • Available in convenient prepacked HisPrep and HisTrap column formats.
  • Composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix and pre-charged with Ni2+ ions for purification of histidine tagged proteins.
  • The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni2+ leakage while providing high capacity.
  • Available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
  • As member of the BioProcess media range, Ni Sepharose 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.