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Cytiva Ni Sepharose™ 6 Fast Flow

Combines the advantages of Ni2 for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform.   Cytiva Lifescience™ Ni Sepharose™ 6 Fast Flow is part of a range of IMAC chromatography media for capture and intermediate purification.

Brand:  Cytiva 17-5318-03

Code : GE

Additional Details : Weight : 0.79800kg


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Product Code. 10353060

  • £4808.00 / 500mL
Estimated Shipment: 18-04-2024
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Description

Description

Ni Sepharose 6 Fast Flow is a member of the Cytiva range of IMAC chromatography media for capture and intermediate purification.

  • Designed for scaling up purification of histidine-tagged proteins.
  • High protein binding capacity and minimal leakage of Ni2+ ions.
  • As a BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
  • Available in convenient prepacked HisPrep and HisTrap column formats.
  • Composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix and pre-charged with Ni2+ ions for purification of histidine tagged proteins.
  • The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni2+ leakage while providing high capacity.
  • Available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
  • As member of the BioProcess media range, Ni Sepharose 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Specifications

Specifications

0.1M HCl, 0.1M NaOH, 8M Urea
500mL
250mm
1 bar
90μm
4° to 30°C
Ni Sepharose 6 FF chromatography media
9.84 in.
600cm/hr.
3 to 12
6% Cross-linked Agarose
Ni-Sepharose
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Safety and Handling

Signal Word
  • Warning

Hazard Category
  • Flammable liquid Category 3
  • ACUTE AQUATIC Acute 1
  • LONG-TERM AQUATIC HAZARD Chronic 3

Hazard Statement
  • H226-Flammable liquid and vapour.
  • H400-Very toxic to aquatic life.
  • H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P273-Avoid release to the environment.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

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