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GE Healthcare Ni Sepharose™ 6 Fast Flow

Combines the advantages of Ni2 for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform.   GE Healthcare Lifescience™ Ni Sepharose™ 6 Fast Flow is part of a range of IMAC chromatography media for capture and intermediate purification.

Brand:  GE Healthcare 17-5318-02

Code : GE

Additional Details : Weight : 1.00000kg

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Product Code. 10343060

  • £875.06 / 100mL


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Description

Description

Ni Sepharose 6 Fast Flow is a member of the GE Healthcare range of IMAC chromatography media for capture and intermediate purification.

  • Designed for scaling up purification of histidine-tagged proteins.
  • High protein binding capacity and minimal leakage of Ni2+ ions.
  • As a BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
  • Available in convenient prepacked HisPrep and HisTrap column formats.
  • Composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix and pre-charged with Ni2+ ions for purification of histidine tagged proteins.
  • The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni2+ leakage while providing high capacity.
  • Available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
  • As member of the BioProcess media range, Ni Sepharose 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Specifications

Specifications

0.1M HCl, 0.1M NaOH, 8M Urea
100mL
600cm/hr.
3 to 12
6% Cross-linked Agarose
Ni Sepharose 6 FF chromato graphy media
250mm
1 bar
90μm
4° to 30°C
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Safety and Handling

Safety and Handling

Signal Word
  • Warning

Hazard Category
  • Flammable liquid Category 3

Hazard Statement
  • H226-Flammable liquid and vapour.

Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P235-Keep cool.
  • P501-Dispose of contents/container to .

Special Offers

Special Offers

  • Use code 13239 to receive this promotion.
    : 13239

    2018-08-31

    *Discount will apply to qualifying orders received by Fisher Scientific between 15/05/2018 and 31/08/2018 subject to availability of the product in stock. Promotion code must be presented at the time of order. Discount applies to the list price in effect at the time the order is received. Promotion cannot be combined with other discounts or promotions. Offer void where prohibited, licensed, or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy or if the product is no longer available in stock. Other restrictions may apply.

     

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