GE Healthcare HiTrap™ Chelating HP Columns

Optimize histidine-tagged protein purifications when nickel ions are not the best choice. GE Healthcare Life Sciences™ HiTrap™ Chelating HP Columns is prepacked with Chelating Sepharose™ High Performance.

Brand: GE Healthcare

Manufacturer Part Number: 17-0409-03

UNSPSC: 41116104

Code: GE

Additional Details:
Additional Details: Weight: 2.26800kg

Product Code. 11594895

Quantity Price
1 £ 471.59000000000003 / Pack of 5
In Stock 3
Add to basket

If this item is out of stock, delivery will be in 3-4 working days providing the item is in stock at the supplier.
Please enquire with customer services on 01509 555500 for stock availability.


Description and Specification


Flow Rate <20mL/min.
Storage Requirements 4° to 30°C
Diameter (Metric) Inner 16mm
Product Type HiTrap chelating HP column
Material Polypropylene
Buffer 6M Guanidine Hydrochloride, 8M Urea
Ligand Type Iminodiacetic Acid
Particle Size 34μm
For Use With (Application) IMAC and subsequent purification of Polyhistidine tagged Proteins
Support Type 6% Highly Cross-linked Agarose
pH Range 3 to 13
Bed Volume 5mL
Height (Metric) 25mm
Quantity 5 x 5mL
Max. Pressure 3 bar

  • Uses different kinds of metal ions
  • Binding capacity ∽12mg histidine-tagged protein/mL medium
  • Metal ion capacity ∽23μmol Cu2+/mL medium
  • Prepacked with Chelating Sepharose High Performance media
  • Iminoacetic acid coupled to a 6% agarose matrix by ether linkage