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  6. Cytiva HiTrap™ Chelating HP Columns

Cytiva HiTrap™ Chelating HP Columns

Product Code. 11594895 Shop All Cytiva Products
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Quantity:
1 x 5mL
5 x 1mL
5 x 5mL
100 x 5 mL
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Product Code. Quantity unitSize
11594895 5 x 5mL Pack of 5
11850073 100 x 5 mL Pack of 100
10773017 1 x 5mL Each
10605575 5 x 1mL Pack of 5
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Product Code. 11594895 Supplier Cytiva Supplier No. 17040903

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Description

Optimize histidine-tagged protein purifications when nickel ions are not the best choice. Cytiva Life Sciences™ HiTrap™ Chelating HP Columns is prepacked with Chelating Sepharose™ High Performance.

  • Uses different kinds of metal ions
  • Binding capacity ∽12mg histidine-tagged protein/mL medium
  • Metal ion capacity ∽23μmol Cu2+/mL medium
  • Prepacked with Chelating Sepharose High Performance media
  • Iminoacetic acid coupled to a 6% agarose matrix by ether linkage

TRUSTED_SUSTAINABILITY

Specifications

Buffer 6M Guanidine Hydrochloride, 8M Urea
Product Type HiTrap chelating HP column
Bed Volume 5mL
Diameter (English) Inner 0.62 in.
Diameter (Metric) Inner 16mm
Quantity 5 x 5mL
Height (English) 0.98 in.
Height (Metric) 25mm
Flow Rate <20 mL/min.
Material Polypropylene
Ligand Type Iminodiacetic Acid
Max. Pressure 3 bar
pH Range 3 to 13
Particle Size 34μm
Support Type 6% Highly Cross-linked Agarose
Content And Storage 4° to 30°C
Column Type Affinity Column
For Use With (Application) IMAC and subsequent purification of Polyhistidine tagged Proteins
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Safety and Handling

compliance-icons
Product Identifier
  • HiTrap Chelating HP 5 ml, 5 x 5 ml
 Signal Word
  • Warning
 Hazard Category
  • Flammable liquid Category 3
 Hazard Statement
  • H226-Flammable liquid and vapour.
 Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P235-Keep cool.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contain: ethanol
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