GE Healthcare HiTrap™ Chelating HP Columns

Optimize histidine-tagged protein purifications when nickel ions are not the best choice. GE Healthcare Life Sciences™ HiTrap™ Chelating HP Columns is prepacked with Chelating Sepharose™ High Performance.

Brand: GE Healthcare

Manufacturer Part Number: 17-0409-03

X5 HITrap Chelating High performance 5mL

UNSPSC: 41116104

Code: GE

Additional Details:
Additional Details: Weight: 2.26800kg

Product Code. 11594895

Quantity Price
1 £ 471.59000000000003 / Pack of 5
Estimated Shipment
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Description and Specification


Flow Rate <20mL/min.
Storage Requirements 4° to 30°C
Diameter (Metric) Inner 16mm
Material Polypropylene
Product Type HiTrap chelating HP column
Buffer 6M Guanidine Hydrochloride, 8M Urea
Ligand Type Iminodiacetic Acid
Particle Size 34μm
Quantity 5 x 5mL
For Use With (Application) IMAC and subsequent purification of Polyhistidine tagged Proteins
Height (Metric) 25mm
pH Range 3 to 13
Support Type 6% Highly Cross-linked Agarose
Bed Volume 5mL
Max. Pressure 3 bar

  • Uses different kinds of metal ions
  • Binding capacity ∽12mg histidine-tagged protein/mL medium
  • Metal ion capacity ∽23μmol Cu2+/mL medium
  • Prepacked with Chelating Sepharose High Performance media
  • Iminoacetic acid coupled to a 6% agarose matrix by ether linkage