GE Healthcare HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. GE Healthcare Life Sciences™ HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Brand: GE Healthcare

Manufacturer Part Number: 17-5247-01


UNSPSC: 41116104

Code: GE

Additional Details:
Additional Details: Weight: 0.00500kg

Product Code. 10431065

Quantity Price
1 £125.21 / 5mL
In Stock 7
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Description and Specification


Bed Volume 1mL
Diameter (Metric) Inner 7mm
Flow Rate 4mL/min.
Material Polypropylene
Packaging Quantity 5 x 1mL
pH Range 3 to 12
Product Type HisTrap FF Crude Column
Pressure 3 bar
Quantity 5 x 1mL
Storage Requirements 4° to 30°C
Support Type 6% Highly Cross-linked Agarose

  • Prepacked with Ni Sepharose™ High Performance medium
  • Negligible nickel ion leakage
  • Pressure Max: 3 bar
  • Column material: polypropylene
  • 1 and 5mL columns
  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
  • Manual operation with syringe, pump or chromatography system

  • Matrix: 6% cross-linked agarose
  • Average particle size: 34μm
  • pH stability working range: 3 to 12

1mL columns

  • Bed Dimensions: 7 x 25mm
  • Flow velocity: 4mL/min.

5mL columns
  • Bed Dimensions: 16 x 25mm
  • Flow velocity: 5mL/min.