GE Healthcare HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. GE Healthcare Life Sciences™ HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Overview
Brand: GE Healthcare

Manufacturer Part Number: 17524701

UNSPSC: 41116104

Code: GE

Additional Details:
Additional Details: Weight: 0.09800kg



Product Code. 10431065

Quantity Price
1 £ 141.4 / 5mL
In Stock 12
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If this item is out of stock, delivery will be in 3-4 working days providing the item is in stock at the supplier.
Please enquire with customer services on 01509 555500 for stock availability.

 

Description and Specification

Specification

Diameter (Metric) Inner 7mm
Storage Requirements 4° to 30°C
Flow Rate 4mL/min.
Material Polypropylene
Product Type HisTrap FF Crude Column
Packaging Quantity 5 x 1mL
Support Type 6% Highly Cross-linked Agarose
Quantity 5 x 1mL
pH Range 3 to 12
Bed Volume 1mL
Pressure 3 bar

  • Prepacked with Ni Sepharose™ High Performance medium
  • Negligible nickel ion leakage
  • Pressure Max: 3 bar
  • Column material: polypropylene
  • 1 and 5mL columns
  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
  • Manual operation with syringe, pump or chromatography system

Media
  • Matrix: 6% cross-linked agarose
  • Average particle size: 34μm
  • pH stability working range: 3 to 12

1mL columns

  • Bed Dimensions: 7 x 25mm
  • Flow velocity: 4mL/min.

5mL columns
  • Bed Dimensions: 16 x 25mm
  • Flow velocity: 5mL/min.