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GE Healthcare HisTrap™ FF Crude Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cell lysates. GE Healthcare Life Sciences™ HisTrap™ FF Crude Prepacked Columns offer the convenience of simple, one-step purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Brand:  GE Healthcare 17-5286-01

Code : GE

Additional Details : Weight : 0.14600kg

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Product Code. 11723219

  • £445.21 / 25mL


In Stock 4
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If this item is out of stock, delivery will be in 3-4 working days providing the item is in stock at the supplier.
Please enquire with customer services on 01509 555500 for stock availability.

 

Description

Description

  • Prepacked with Ni Sepharose™ 6 Fast Flow media
  • Binding capacity approximately 40mg/mL medium with negligible Ni2+ leakage
  • Maximum pressure: 3 bar
  • Column material: polypropylene
  • Compatible with wide variety of reducing agents, detergents, denaturants, and other additives
  • 1 and 5mL columns

Media
  • Matrix: 6% cross-linked agarose
  • Average particle size: 90μm
  • pH stability working range: 3 to 12

1mL columns

  • Bed dimensions: 7 x 25mm
  • Flow velocity: <4mL/min.

5mL columns
  • Bed dimensions: 16 x 25mm
  • Flow velocity: <20mL/min.

Specifications

Specifications

5mL
<20mL/min.
5 x 5mL
45 to 165μm
3 bar
4° to 30°C
16mm
Polypropylene
3 to 12
HisTrap FF Crude Column
5 x 5mL
6% Cross-linked Agarose
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Safety and Handling

Safety and Handling

Signal Word
  • Warning

Hazard Category
  • Flammable liquid Category 3

Hazard Statement
  • H226-Flammable liquid and vapour.

Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/A§/ equipment.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P235-Keep cool.
  • P501-Dispose of contents/container to A§.