GE Healthcare HisTrap™ FF Crude Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cell lysates. GE Healthcare Life Sciences™ HisTrap™ FF Crude Prepacked Columns offer the convenience of simple, one-step purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Overview
Brand: GE Healthcare

Manufacturer Part Number: 17-5286-01

X5 HISTrap Fast flow crude column

UNSPSC: 41116104

Code: GE

Additional Details:
Additional Details: Weight: 0.14600kg



Product Code. 11723219

Quantity Price
1 £ 424.2 / 25mL
In Stock 8
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Description and Specification

Specification

Bed Volume 5mL
Diameter (Metric) Inner 16mm
Flow Rate <20mL/min.
Material Polypropylene
Packaging Quantity 5 x 5mL
pH Range 3 to 12
Particle Size 45 to 165μm
Product Type HisTrap FF Crude Column
Pressure 3 bar
Quantity 5 x 5mL
Storage Requirements 4° to 30°C
Support Type 6% Cross-linked Agarose

  • Prepacked with Ni Sepharose™ 6 Fast Flow media
  • Binding capacity approximately 40mg/mL medium with negligible Ni2+ leakage
  • Maximum pressure: 3 bar
  • Column material: polypropylene
  • Compatible with wide variety of reducing agents, detergents, denaturants, and other additives
  • 1 and 5mL columns

Media
  • Matrix: 6% cross-linked agarose
  • Average particle size: 90μm
  • pH stability working range: 3 to 12

1mL columns

  • Bed dimensions: 7 x 25mm
  • Flow velocity: <4mL/min.

5mL columns
  • Bed dimensions: 16 x 25mm
  • Flow velocity: <20mL/min.