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  6. Cytiva HisTrap™ FF Crude Prepacked Columns

Cytiva HisTrap™ FF Crude Prepacked Columns

Product Code. 11723219 Shop All Cytiva Products
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Quantity:
5 x 5 mL
60 mL
100 x 1 mL
100 x 5 mL
Flow Rate:
<20 mL/min.
<4 mL/min.
Unit Size:
Pack of 100
Pack of 5
4 product options available for selection
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Product Code. Quantity Flow Rate unitSize
11723219 5 x 5 mL <20 mL/min. Pack of 5
10685355 60 mL <4 mL/min. Pack of 5
11515674 100 x 1 mL <4 mL/min. Pack of 100
11800083 100 x 5 mL <20 mL/min. Pack of 100
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Product Code. 11723219 Supplier Cytiva Supplier No. 17528601

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Description

Purify histidine-tagged proteins directly from homogenized, unclarified cell lysates. Cytiva Life Sciences™ HisTrap™ FF Crude Prepacked Columns offer the convenience of simple, one-step purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

  • Prepacked with Ni Sepharose™ 6 Fast Flow media
  • Binding capacity approximately 40mg/mL medium with negligible Ni2+ leakage
  • Maximum pressure: 3 bar
  • Column material: polypropylene
  • Compatible with wide variety of reducing agents, detergents, denaturants, and other additives
  • 1 and 5mL columns

Media
  • Matrix: 6% cross-linked agarose
  • Average particle size: 90μm
  • pH stability working range: 3 to 12

1mL columns

  • Bed dimensions: 7 x 25mm
  • Flow velocity: <4mL/min.

5mL columns
  • Bed dimensions: 16 x 25mm
  • Flow velocity: <20mL/min.

TRUSTED_SUSTAINABILITY

Specifications

Bed Volume 5 mL
Diameter (English) Inner 0.6 in.
Diameter (Metric) Inner 16 mm
Description HisTrap FF Crude columns are high binding capacity IMAC columns for his-tagged protein purification when scalability is needed.
Flow Rate <20 mL/min.
Material Polypropylene
pH Range 3 to 12
Support Type 6% Cross-linked Agarose
Content And Storage 4°C to 30°C
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
Particle Size 45 to 165 μm
Quantity 5 x 5 mL
For Use With (Application) Intended for research use only, and shall not be used in any clinical or in vitro procedures for diagnostic purposes
Type Pre-packed Column
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Safety and Handling

compliance-icons
Signal Word
  • Warning
 Hazard Category
  • Flammable liquid Category 3
 Hazard Statement
  • H226-Flammable liquid and vapour.
  • EUH208-Contains Nickel. May produce an allergic reaction.
 Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/and all material-handling/ equipment.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P235-Keep cool.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contain: ethanol
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