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GE Healthcare HisTrap™ FF Columns: 16 × 25mm

Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). GE Healthcare Life Sciences™ HisTrap™ FF Columns: 16 × 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

Brand:  GE Healthcare 17-5319-01

UNSPSC : 41116104

Code : GE

Additional Details : Weight : 0.50000kg

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Product Code. 10126414

  • £113.78 / Pack of 5


In Stock 5
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If this item is out of stock, delivery will be in 3-4 working days providing the item is in stock at the supplier.
Please enquire with customer services on 01509 555500 for stock availability.

 

Description

Description

  • Prepacked with precharged Ni Sepharose™ 6 Fast Flow media
  • Column material: polypropylene
  • Bed dimensions: 16 × 25mm
  • Flow velocity: <20mL/min.
  • Protein binding capacity of ∽40mg/mL media with negligible nickel ion leakage
  • Compatible with wide range of reducing agents, detergents, denaturants, and other additives
  • Simple operation with syringe, perstaltic pump, or chromatographic system
  • Columns may be connected in series to increase capacity

Medium
  • Matrix: 6% cross-linked agarose
  • Average particle size: 90μm
  • pH stability working range: 3 to 12

Specifications

Specifications

1mL
<20mL/min.
5 × 1mL
HisTrap Column
5 × 1mL
6% Cross-linked Agarose
16mm
Polypropylene
3 to 12
3 bar
4° to 30°C in 20% ethanol
Safety and Handling

Safety and Handling

Signal Word
  • Warning

Hazard Category
  • Flammable liquid Category 3

Hazard Statement
  • H226-Flammable liquid and vapour.

Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P235-Keep cool.
  • P501-Dispose of contents/container to .