GE Healthcare HisTrap™ FF Columns: 16 × 25mm

Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). GE Healthcare Life Sciences™ HisTrap™ FF Columns: 16 × 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

Brand: GE Healthcare

Manufacturer Part Number: 17-5319-01


UNSPSC: 41116104

Code: GE

Additional Details:
Additional Details: Weight: 0.09900kg

Product Code. 10126414

Quantity Price
1 £105.37 / Pack of 5
In Stock 6
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Description and Specification


Bed Volume 1mL
Diameter (Metric) Inner 16mm
Flow Rate <20mL/min.
Material Polypropylene
Packaging Quantity 5 × 1mL
pH Range 3 to 12
Product Type HisTrap Column
Pressure 3 bar
Quantity 5 × 1mL
Storage Requirements 4° to 30°C in 20% ethanol
Support Type 6% Cross-linked Agarose

  • Prepacked with precharged Ni Sepharose™ 6 Fast Flow media
  • Column material: polypropylene
  • Bed dimensions: 16 × 25mm
  • Flow velocity: <20mL/min.
  • Protein binding capacity of ∽40mg/mL media with negligible nickel ion leakage
  • Compatible with wide range of reducing agents, detergents, denaturants, and other additives
  • Simple operation with syringe, perstaltic pump, or chromatographic system
  • Columns may be connected in series to increase capacity

  • Matrix: 6% cross-linked agarose
  • Average particle size: 90μm
  • pH stability working range: 3 to 12