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Cytiva HiScreen™ Blue, Ni and DEAE Sepharose™ Prepacked Fast Flow Columns
Used for method optimization and parameter screening
Brand: Cytiva 28-9782-45
Code : GX
Additional Details : Weight : 0.08300kg
Description
- Based on 6% highly cross-linked agarose with an average bead size of 90μm
- Processed flow properties and high loading capacities
- Bed height of 10cm is designed to allow method optimization and parameter screening
- Easily connected in series to achieve 20cm bed height
- Small bed volume gives fast results and minimal sample/buffer consumption
- Combines high capacity with high flow velocity and low backpressure to reduce process cycle times and increase productivity
- Reproducible results, scalable to BioProcess™ columns packed with the same chromatography media using the same linear fluid velocity
- Bed volume: 4.7mL
- Bed dimension: 7.7 × 100mm
- Matrix: 6% cross-linked agarose beads
- Average particle size: 90μm
- Flow velocity: <600cm/hr or <450cm/hr
- Material (Column hardware and column tube): polypropylene
- Column I.D.: 7.7mm
Blue Sepharose
- Prepacked with BioProcess chromatography medium Blue Sepharose 6 Fast Flow
- Purification of albumin, enzymes (including NAD+ and NADP+), coagulation factors, interferons and related proteins
- Prepacked with Nickel Sepharose 6 Fast Flow
- Purification of histidine-tagged proteins with Immobilized Metal ion Affinity Chromatography (IMAC)
- Prepacked with DEAE Sepharose Fast Flow
- Weak anion exchanger (diethylaminoethyl group) BioProcess™ medium
Specifications
| 1M NaOH, 8M Urea, 8M Guanidine Hydrochloride, 70% Ethanol | |
| 4.7mL | |
| 7.7mm | |
| 3.93 in. | |
| <450cm/hr. | |
| Diethylaminoethyl | |
| 2 to 12 | |
| 6% Cross-linked Agarose | |
| Ion Exchange Column |
| HiScreen DEAE Sepharose FF column | |
| 0.3 in. | |
| 1 x 4.7mL | |
| 100mm | |
| Polypropylene | |
| 1.5 bar | |
| 45 to 165μm | |
| 4° to 30°C | |
| Convenient process development optimization |