GE Healthcare HiScreen™ Blue, Ni and DEAE Sepharose™ Prepacked Fast Flow Columns

Used for method optimization and parameter screening

Brand: GE Healthcare

Manufacturer Part Number: 28-9782-45

HiScreen DEAE FF, 1 x 4.7 ml

UNSPSC: 41115700

Code: GX

Additional Details:
Additional Details: Weight: 0.08300kg

Product Code. 11370322

Quantity Price
1 £ 73.29 / Each
Estimated Shipment
Add to basket

If this item is out of stock, delivery will be in 3-4 working days providing the item is in stock at the supplier.
Please enquire with customer services on 01509 555500 for stock availability.


Description and Specification


Buffer 1M NaOH, 8M Urea, 8M Guanidine Hydrochloride, 70% Ethanol
Bed Volume 4.7mL
Diameter (Metric) Inner 7.7mm
Product Type HiScreen DEAE Sepharose FF column
Quantity 1 x 4.7mL
For Use With (Application) Convenient process development optimization
Height (Metric) 100mm
Flow Rate <450cm/hr.
Material Polypropylene
Ligand Type Diethylaminoethyl
Max. Pressure 1.5 bar
pH Range 2 to 12
Particle Size 45 to 165μm
Support Type 6% Cross-linked Agarose
Storage Requirements 4° to 30°C

  • Based on 6% highly cross-linked agarose with an average bead size of 90μm
  • Processed flow properties and high loading capacities
  • Bed height of 10cm is designed to allow method optimization and parameter screening
  • Easily connected in series to achieve 20cm bed height
  • Small bed volume gives fast results and minimal sample/buffer consumption
  • Combines high capacity with high flow velocity and low backpressure to reduce process cycle times and increase productivity
  • Reproducible results, scalable to BioProcess™ columns packed with the same chromatography media using the same linear fluid velocity

  • Bed volume: 4.7mL
  • Bed dimension: 7.7 × 100mm
  • Matrix: 6% cross-linked agarose beads
  • Average particle size: 90μm
  • Flow velocity: <600cm/hr or <450cm/hr
  • Material (Column hardware and column tube): polypropylene
  • Column I.D.: 7.7mm

Blue Sepharose

  • Prepacked with BioProcess chromatography medium Blue Sepharose 6 Fast Flow
  • Purification of albumin, enzymes (including NAD+ and NADP+), coagulation factors, interferons and related proteins
Ni Sepharose
  • Prepacked with Nickel Sepharose 6 Fast Flow
  • Purification of histidine-tagged proteins with Immobilized Metal ion Affinity Chromatography (IMAC)
DEAE Sepharose
  • Prepacked with DEAE Sepharose Fast Flow
  • Weak anion exchanger (diethylaminoethyl group) BioProcess™ medium