Cytiva His Mag Sepharose™ excel Chromatography Media
His Mag Sepharose excel magnetic beads are designed for small-scale purification and screening of histidine-tagged proteins secreted in eukaryotic cell culture.
Brand: Cytiva 17371222
Code : GX
Additional Details : CAS Number : 79-06-1 Weight : 0.11200kg
Samples that usually cause stripping of metal ions can therefore be added to the medium with retained protein-binding capacity and without pretreatment.
- His Mag Sepharose excel is a magnetic IMAC medium precharged with nickel ions that are very strongly bound to a chelating ligand.
- The medium enables an efficient and simplified workflow for capture, purification, and reproducible screening of histidine-tagged proteins.
- Simplify small-scale purification and expression screening of histidine-tagged proteins secreted in eukaryotic cell culture.
- Purify histidine-tagged proteins directly from samples that contain metal ion-stripping agents and save time and effort.
- Increase target protein yield and decrease degradation through reduced and simplified sample handling.
- Screen histidine-tagged proteins in parallel with high reproducibility and reliable results.
- Capture histidine-tagged protein from different sample volumes; from low to high milliliter scale.
- Simplify handling using visible and dense Sepharose-based magnetic beads.
- Proteins are expressed and secreted into cell culture supernatants from eukaryotic cells such as insect cells or Chinese Hamster Ovary (CHO) cells.
- The high density of the beads allows rapid capture by magnetic devices while the visibility of the beads ensures reliable collection of the bound histidine-tagged proteins during the purification procedure.
- The base matrix is based on Sepharose medium with magnetite incorporated.
- His Mag Sepharose is available as 10% medium slurry in three different pack sizes (2 x 1ml, 5 x 1ml, and 10 x 1ml) and can be used together with different test tubes and magnetic racks.
|2 to 12|
|10 x 1mL|
|Highly cross-linked spherical Agarose including Magnetite|
|Designed to simplify small-scale purification and screening of His-tagged Proteins_x000D_ secreted in eukaryotic cell culture, used for functional and structural studies research in academia, institutes and discovery Pharma|
|His Mag Sepharose Excel|
|4° to 30°C|
Safety and Handling
- Flammable liquid Category 3
- H226-Flammable liquid and vapour.
- EUH208-Contains Nickel. May produce an allergic reaction.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/and all material-handling/ equipment.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P235-Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : ethanol
- UNKNOWN AQUATIC-Contains 50% of components with unknown hazards to the aquatic environment.