Featuring excellent optical performance, the Fluoroskan Microplate Fluorometer is ideal for life science research applications such as fluorometric protein and enzyme studies, molecular interaction studies, nucleic acid quantification, as well as reporter gene, fluorometric kinase, immunological, and cell-based assays.
• High sensitivity for both top and bottom reading
• Onboard dispensers for precise initiation of kinetic reactions
• Powerful and easy-to-use Thermo Scientific SkanIt software
High sensitivity for both top and bottom reading
The use of fiberless direct illumination optics allows for both top and bottom reading enables high sensitivity, a wide dynamic range, and accurate and precise results for 6- to 384-well plates.
Onboard dispensers for precise initiation of kinetic reactions
The Fluoroskan Microplate Fluorometer can be equipped with up to three reagent dispensers* for use during fast kinetic assays, such as Ca2+ flux assays. The onboard dispensers support simultaneous dispensing and reading, enabling the monitoring of fast kinetic measurements from the very start of the reaction. Very low dead volume and backflush of reagents support conservation of reagents.
* Upgrade to third dispenser must be performed by service technician
Powerful and easy-to-use SkanIt software
With a visual workflow setup, SkanIt software is quick and easy to learn. It offers easy data analysis (with a comprehensive selection of built-in calculations, including blank subtraction, standard curve creation, classification, quality control, kinetic analysis) and exporting capabilities. Each user can install SkanIt software on his/her own computer to set up assays and analyze data.
SkanIt PC Software and the following filter pairs (excitation/emission): 355 nm/460 nm, 485 nm/538 nm. An extensive selection of additional filters is available upon request.
Ca2+ flux assays, cell proliferation, cytotoxicity, cell adhesion, nucleic acid quantitation, reporter gene assays, hybridization assays, ion channel, FRET assays, immunoassays, enzyme activity, bacterial quantitation, phagocytosis, oligonucleotide assays
|420 x 420 x 340 mm (16.5 x 16.5 x 13.4 in.)|
|PC Software (Thermo Scientific SkanIt Software)|
|18 s for 96-well Plate|
|6- to 384-well Plates|
|Top and Bottom|
|Photomultiplier Tube (PMT)|
|Ambient +3°C to 45°C|
|Quartz Halogen Lamp|
|Up to approx. 5000 Relative Fluorescence Units (RFU)|
|200 VA max.|