missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Crosslinking, Labeling and Protein Modification
  5. Protein and Antibody Labeling Kits
  6. 10462282

Molecular Probes™ FluoReporter™ Biotin Quantitation Assay Kit, for biotinylated proteins

Product Code. 10462282
Click to view available options
Quantity:
1 Kit
Unit Size:
1 set
This item is not returnable. View return policy
For Research Use Only. All usage must comply with product instructions.
 

Product Code. 10462282

Brand: Molecular Probes™ F30751

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy
For Research Use Only. All usage must comply with product instructions.
 

Description

FluoReporter™ Biotin Quantitation Assay Kit, for biotinylated proteins

The FluoReporter™ Biotin Quantitation Assay Kit provides a sensitive fluorometric assay for determining the number of biotin labels on nucleic acid samples (i.e., cDNA). The assay uses Biotective™ Green reagent, which contains a fluorescent dye that is quenched by ligands occupying the biotin binding sites. In the presence of biotin, the quencher dye ligands are displaced and fluorescence occurs. The fluorescence is proportional to the amount of added biotin. The results can be read with any fluorescence-based microplate reader capable of detecting FITC dye.

Important Features of FluoReporter™ Biotin Quantitation Assay Kits:
  • Can detect from 4 to 80 picomoles of biotin in a sample (50-fold higher sensitivity than HABA biotin-binding assay)
  • Can be applied to as little as 13 ng of biotin-labeled nucleic acid (more is needed for lower degrees of labeling)
  • Excitation/emission maxima of the reagent is 495/519 nm
  • Supplied with a biotinylated positive control for standard curve

    For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
    • TRUSTED_SUSTAINABILITY

    Specifications

    Product Type Biotin Quantitation Assay Kit
    Quantity 1 Kit
    Content And Storage Store in freezer (-5 to -30°C) and protect from light.
    Detection Method Fluorescence
    Shipping Condition Room Temperature
    Product Line FluoReporter™
    Label or Dye Biotin
    Labeling Target Proteins

    For Research Use Only. Not for use in diagnostic procedures.

    Product Content Correction

    Your input is important to us. Please complete this form to provide feedback related to the content on this product.

    Product Title

    By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

    Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.