Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 780

Product Code.	Quantity	unitSize
13539140	100 Tests	100 tests
15383562	500 Tests	500 tests

Product Code. 13539140 Supplier Invitrogen™ Supplier No. 65086514

Description

A viability dye that can be used to irreversibly label dead cells prior to cryopreservation, fixation, and/or permeabilization procedures.

• Unlike 7-AAD and propidium iodide, cells labeled with Fixable Viability Dyes can be washed, fixed, permabilized, and stained for intracellular antigens without any loss of staining intensity of the dead cells
• Thus, using Fixable Viability Dyes allows dead cells to be excluded from analysis when intracellular targets are being studied
• Fixable Viability Dye eFluor™ 780 can be excited by the red (633 nm) laser line and has a peak emission of 780 nm that can be detected using a 780/60 band pass filter (equivalent to APC-eFluor™ 780 or APC-Alexa Fluor™ 750)
• For compensation, it is recommended to use a sample of the cells of interest stained with the Fixable Viability Dye
• If the percentage of dead cells is expected to be less than 5%, then it is recommended to take a small aliquot of cells and heat them at 65°C for 1 minute then immediately place on ice for 1 minute
• After this treatment, the heat-killed cells can be combined 1:1 with live cells and then stained with the Fixable Viability Dye
• Testing suggests that compensation out of most detectors is negligible, with compensation out of PE-Cyanine7 being the highest at less than 5%
• Actual compensation values will depend on each investigator's specific instrument, filter sets, and PMT voltage settings
• Fixable Viability Dye eFluor™ 780 is supplied as a pre-diluted solution prepared in high-quality, anhydrous DMSO
• It should be protected from light and moisture
• Store at -70°C with dessicant, it may be freeze-thawed up to 20 times
• Allow vial to equilibrate to room temperature before opening

Specifications

• Color: Infrared
• Detection Method: Fluorescence
• For Use With (Application): Viability Assay
• For Use With (Equipment): Flow Cytometer
• Product Type: Dye
• Emission: 633/780
• Product Line: eBioscience
• Quantity: 100 Tests

Frequently Asked Questions (FAQs)

How do I discriminate between live cells and dead cells when doing a flow cytometry analysis?

Discrimination between viable and non-viable cells can be carried out with the use of the 7-AAD Viability Dye (Cat. No. 00-6993-50) or Propidium Iodide (PI) Staining Solution (Cat. No. 00-6990-50). The 7-AAD or PI will mark the non-viable cells by binding to the nuclei of those cells. The nucleic acid of viable cells will not be accessible to the dye and will not be stained. When analyzing the data collected, gate out all cells stained with the viability dye. When staining for intracellular proteins, use the Fixable Viability Dye eFluor 455UV, 450, 520, 660, or 780.

Can Fixable Viability Dyes be used with the eBioscience BrdU Staining kits for Flow Cytometry?

Yes, a Fixable Viability Dye can be added to samples after BrdU labeling and before fixation with the BrdU Staining Buffer. See the protocol for additional information.

For Research Use Only. Not for use in diagnostic procedures.