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Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 506

A viability dye that can be used to irreversibly label dead cells prior to cryopreservation, fixation, and/or permeabilization procedures.

Brand:  Invitrogen™ 65-0866-14

64.50 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15560607

  • £96.75 / 100 tests
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Description

Description

  • Unlike 7-AAD and propidium iodide, cells labeled with Fixable Viability Dyes can be washed, fixed, permabilized, and stained for intracellular antigens without any loss of staining intensity of the dead cells
  • Thus, using Fixable Viability Dyes allows dead cells to be excluded from analysis when intracellular targets are being studied
  • Fixable Viability Dye eFluor™ 506 can be excited by the violet (405 nm) laser line and has a peak emission of 506 nm that can be detected using a 510/50 band pass filter (equivalent to AmCyan)
  • For compensation, it is recommended to use a sample of the cells of interest stained with the Fixable Viability Dye
  • If the percentage of dead cells is expected to be less than 5%, then it is recommended to take a small aliquot of cells and heat them at 65°C for 1 minute then immediately place on ice for 1 minute
  • After this treatment, the heat-killed cells can be combined 1:1 with live cells and then stained with the Fixable Viability Dye
  • Fixable Viability Dye eFluor™ 506 is supplied as a pre-diluted solution prepared in high-quality, anhydrous DMSO
  • It should be protected from light and moisture
  • Store at less than or equal to -70°C with dessicant, it may be freeze-thawed up to 20 times
  • Allow vial to equilibrate to room temperature before opening
Specifications

Specifications

Cyan
Viability Assay
Dye
eBioscience™
Fluorescent
Flow Cytometer
405/506
100 Tests
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For Research Use Only. Not for use in diagnostic procedures.