missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 450
A viability dye that can be used to irreversibly label dead cells prior to cryopreservation, fixation, and/or permeabilization procedures.
£103.00 - £298.00
Specifications
Color | Blue |
---|---|
Detection Method | Fluorescent |
For Use With (Application) | Viability Assay |
For Use With (Equipment) | Flow Cytometer |
Product Type | Dye |
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
---|---|---|---|---|---|---|---|---|---|
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
15211938
|
Invitrogen™
65-0863-14 |
100 Tests |
£103.00
Each |
In Stock Log in to see stock. |
Please sign in to purchase this item. Need a web account? Register with us today! | ||||
15331988
|
Invitrogen™
65-0863-18 |
500 Tests |
£298.00
Pack of 500 |
In Stock Log in to see stock. |
Please sign in to purchase this item. Need a web account? Register with us today! | ||||
Description
- Unlike 7-AAD and propidium iodide, cells labeled with Fixable Viability Dyes can be washed, fixed, permabilized, and stained for intracellular antigens without any loss of staining intensity of the dead cells
- Thus, using these dyes allows dead cells to be excluded from analysis when intracellular targets are being studied
- Fixable Viability Dye eFluor™ 450 can be excited by the violet (405 nm) laser line and has a peak excitation of 450 nm that can be detected using a 450/50 band pass filter (equivalent to eFluor™ 450 or Pacific Blue™)
- For compensation, it is recommended to use a sample of the cells of interest stained with the Fixable Viability Dye
- If the percentage of dead cells is expected to be less than 5%, then it is recommended to take a small aliquot of cells and heat them at 65°C for 1 minute then immediately place on ice for 1 minute
- After this treatment, the heat-killed cells can be combined 1:1 with live cells and then stained with the Fixable Viability Dye
- Fixable Viability Dye eFluor™ 450 is supplied as a pre-diluted solution prepared in high-quality, anhydrous DMSO
- It should be protected from light and moisture
- Store at -70°C with dessicant, it may be freeze-thawed up to 20 times
- Allow vial to equilibrate to room temperature before opening
Specifications
Blue | |
Viability Assay | |
Dye | |
eBioscience™ |
Fluorescent | |
Flow Cytometer | |
405/450 |
For Research Use Only. Not for use in diagnostic procedures.