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Thermo Scientific™ EZ-Link™ Sulfo NHS-SS Biotinylation Kit

Product Code. 10157723 Shop All Thermo Scientific Products
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Quantity:
10 Reactions
Unit Size:
1 set
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Product Code. 10157723

Brand: Thermo Scientific™ 21445

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Description

Biotinylate protein amines with this cleavable, mid-length (24.3A), water-soluble, NHS-ester biotinylation reagent containing a cleavable disulfide bond in the spacer arm.

Thermo Scientific™ EZ-Link Sulfo-NHS-SS-Biotin enables simple and efficient biotin labeling of antibodies, proteins and any other primary amine-containing biomolecules in solution.

Highlights:

  • Cleavable analog of Sulfo-NHS-LC-Biotin
  • Can remove biotin label by cleaving spacer disulfide bond with reducing agents, such as DTT
  • Ideal for affinity purification experiments that require efficient elution from immobilized avidin supports (cleaving disulfide bond releases proteins)
  • Long spacer arm reduces steric hindrance for binding interactions
TRUSTED_SUSTAINABILITY

Specifications

Description EZ-Link Sulfo-NHS-SS-Biotinylation Kit
Product Type Sulfo-NHS-SS Biotinylation Kit
Quantity 10 Reactions
Content And Storage Sufficient For: 10 labeling reactions, each with 1 to 10 mg of antibody
• EZ-Link Sulfo-NHS-SS-Biotin, 25 mg
• PBS Pack (makes 500 mL), 1 pack
• Zeba Spin Desalting Columns (7K MWCO), 5 mL, 10 columns
• HABA Solution, 1 mL
• Avidin, 10 mg

Store avidin at -20°C. Store biotin reagent desiccated at 4°C. Store remaining kit components at 4°C.
Labeling Scale 1 to 10 mg
Product Line EZ-Link
Solubility Water
Label or Dye Biotin
Labeling Target Antibodies, Proteins

Safety and Handling

compliance-icons
Product Identifier
  • EZ-Link Sulfo NHS-SS Biotinylation Kit
 Signal Word
  • Warning
 Hazard Category
  • Serious eye damage/eye irritation Category 2
 Hazard Statement
  • H319-Causes serious eye irritation.
 Precautionary Statement
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P264-Wash hands thoroughly after handling.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
 Supplemental information
  • MIXTURE LIST-Contains : disodium hydrogenorthophosphate sodium chloride

For Research Use Only. Not for use in diagnostic procedures.

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