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Thermo Scientific™ EZ-Link™ Micro Sulfo-NHS-SS-Biotinylation Kit

Product Code. 10055833 Shop All Thermo Scientific Products
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8 reactions
Unit Size:
1 set
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10055833 8 reactions 1 set
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Product Code. 10055833 Supplier Thermo Scientific™ Supplier No. 21945

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Description

Biotinylate protein amines with this cleavable, mid-length (24.3A), water-soluble, NHS-ester biotinylation reagent containing a cleavable disulfide bond in the spacer arm.

Thermo Scientific™ EZ-Link Sulfo-NHS-SS-Biotin enables simple and efficient biotin labeling of antibodies, proteins and any other primary amine-containing biomolecules in solution.

Highlights:

  • Cleavable analog of Sulfo-NHS-LC-Biotin
  • Can remove biotin label by cleaving spacer disulfide bond with reducing agents, such as DTT
  • Ideal for affinity purification experiments that require efficient elution from immobilized avidin supports (cleaving disulfide bond releases proteins)
  • Long spacer arm reduces steric hindrance for binding interactions
TRUSTED_SUSTAINABILITY

Specifications

Description EZ-Link Micro Sulfo-NHS-SS-Biotinylation Kit
Product Type Micro Sulfo-NHS-SS-Biotinylation Kit
Quantity 8 reactions
Content And Storage Sufficient For: 8 labeling reactions, each with 0.05–0.2 mg of antibody
• EZ-Link Sulfo-NHS-SS-Biotin, 8 x 1 mg
• PBS Pack (makes 500 mL), 1 pack
• Zeba Spin Desalting Columns (7K MWCO), 2 mL, 10 columns

Store biotin reagent at -20°C. Store remaining kit components at 4°C.
Labeling Scale 100 μg
Product Line EZ-Link
Solubility Water
Label or Dye Biotin
Labeling Target Antibodies, Proteins

Safety and Handling

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Product Identifier
  • EZ-Link Micro Sulfo-NHS-SS-Biotinylation Kit
 Signal Word
  • Warning
 Hazard Category
  • Serious eye damage/eye irritation Category 2
 Hazard Statement
  • H319-Causes serious eye irritation.
 Precautionary Statement
  • P264-Wash hands thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
 Supplemental information
  • MIXTURE LIST-Contain: Sodium phosphate dibasic, Sodium chloride

For Research Use Only. Not for use in diagnostic procedures.

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