Thermo Scientific™ EZ-Link™ Sulfo-NHS-Biotin and Labeling Kits

Biotinylate protein amines (e.g., lysines) with our shortest (13.5A), water-soluble, NHS-ester biotinylation reagent for antibody or cell-surface labeling.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 21425

SULFO-NHS-BIOTINYLATION KIT, EZ-LINK 00 STORE INFridge at 4C

UNSPSC: 41115824

Code: PN

Additional Details:
Additional Details: Weight: 3.17510kg



Product Code. 10321634

Quantity Price
1 £ 251.0 / 1 set
EU Stock 8
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Description and Specification

Specification

Chemical Reactivity Amine
Cleavable No
Description EZ-Link Sulfo-NHS-Biotinylation Kit
Formulation Multi-component kit with Sulfo-NHS-Biotin
Kit Contents
  • EZ-Link Sulfo-NHS-Biotin, 25mg
  • PBS Pack (makes 500mL), 1 pack
  • Zeba Spin Desalting Columns (7K MWCO), 5mL, 10 columns
  • HABA Solution, 1mL
  • Avidin, 10mg
Molecular Weight 443.43
Membrane Permeable No
PEGylated No
Spacer Arm Length 13.5
Quantity 10 rxn kit
Sufficient For 10 labeling reactions, each with 1 to 10mg of antibody
Storage Requirements -20°C with desiccant
Water Soluble Yes

Highlights:

  • Shorter spacer arm than Sulfo-NHS-LC-Biotin
  • Reacts with primary amines at pH 7-9 in non-amine-containing buffers such as borate, carbonate, or phosphate buffers
  • Negative charge of -SO3 keeps biotinylation localized at the cell surface so reagent does not pass through cell membrane
  • Water solubility eliminates the need for harmful organic solvents
U.S. Patents 5,892,628 and 5,872,261.

Documentation