Thermo Scientific™ EZ-Link™ Sulfo-NHS-Biotinylation Kit

Biotinylate protein amines (e.g., lysines) with our shortest (13.5A), water-soluble, NHS-ester biotinylation reagent for antibody or cell-surface labeling.

Brand:  Thermo Scientific™ 21425

Product Code. 10321634

Description

Label antibodies, proteins and other molecules that have primary amines with Thermo Scientific™ EZ-Link Sulfo-NHS-Biotin, a short-chain, water-soluble biotinylation reagent.

Highlights:

• Shorter spacer arm than Sulfo-NHS-LC-Biotin
• Reacts with primary amines at pH 7-9 in non-amine-containing buffers such as borate, carbonate, or phosphate buffers
• Negative charge of -SO₃ keeps biotinylation localized at the cell surface so reagent does not pass through cell membrane
• Water solubility eliminates the need for harmful organic solvents

U.S. Patents 5,892,628 and 5,872,261.

Specifications

• Description: EZ-Link Sulfo-NHS-Biotinylation Kit
• Content And Storage: Sufficient For: 10 labeling reactions, each with 1 to 10 mg of antibody
  • EZ-Link Sulfo-NHS-Biotin, 25 mg
  • PBS Pack (makes 500mL), 1 pack
  • Zeba Spin Desalting Columns (7K MWCO), 5 mL, 10 columns
  • HABA Solution, 1 mL
  • Avidin, 10 mg
  
  Store avidin and vial of biotin reagent (with desiccant) at -20°C. Store remaining kit components at 4°C.
• Product Line: EZ-Link™
• Label or Dye: Biotin

• Product Type: Sulfo-NHS-Biotinylation Kit
• Labeling Scale: 1 to 10 mg
• Solubility: Water
• Labeling Target: Antibodies, Proteins

Safety and Handling

Product Identifier

• EZ-Link Sulfo-NHS-Biotinylation Kit

Signal Word

• Warning

Hazard Category

• Acute toxicity Category 4
• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H302-Harmful if swallowed.
• H312-Harmful in contact with skin.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H332-Harmful if inhaled.
• H335-May cause respiratory irritation.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P271-Use only outdoors or in a well-ventilated area.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P311-Call a POISON CENTER/doctor/ .
• P312-Call a POISON CENTER/doctor/if you feel unwell.
• P330-Rinse mouth.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P362+P364-Take off contaminated clothing and wash it before reuse.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Sulfo-NHS-Biotin, Sodium chloride, Sodium phosphate dibasic

For Research Use Only. Not for use in diagnostic procedures.