PolarScreen™ PolarScreen™ ER Alpha Competitor Assay, Red

Product Code. 15607858

Brand: PolarScreen™ A15884

Description

PolarScreen™ ER Alpha Competitor Assay, Red provides a sensitive and efficient method for high-throughput, fluorescence polarization-based screening of potential ER alpha ligands.

• The PolarScreen™ ER (Estrogen Receptor) Alpha Competitor Assay Kit, Red, provides a sensitive and efficient method for high-throughput, fluorescence polarization-based screening of potential ER alpha ligands. The kit uses insect cell-expressed, full-length, native (untagged) human estrogen receptor alpha (available separately, Cat. No. A15674) and a novel, high-affinity, fluorescent estrogen ligand (Fluormone EL Red) in a homogenous, mix-and-read assay format.
• This kit produces a red color read-out; a green kit is also available. Both kits suitably determine the IC50s of competitive compounds. Color choice depends on the spectral properties of the compounds to be screened and the available instrumentation.
• Complete & ready-to-use out of the box - all reagents included, just add your test compounds
• High throughput compatible - 800 x 20 μL fluorescence polarization assays in a 384-well plate
• Convenient - available in bulk sizes for larger screening needs

Specifications

• Content And Storage: Contains:
  
  • 1 tube full-length ER Alpha Recombinant Protein (store at -68 to -85°C)
  • 1 tube of Fluormone EL Red Tracer (store at -5 to -30°C)
  • 2 bottles of ER Red Screening Buffer (store at -5 to -30°C)
• Detection Method: Fluorescence
• For Use With (Equipment): Fluorescent Imager
• Format: 384-well Plate
• Quantity: 800 assays
• Type: ER Alpha Competitor Assay

For Research Use Only. Not for use in diagnostic procedures.