EGLN1/PHD2 Antibody, Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 74 publications

Brand:  Novus Biologicals NB100-137

Product Code. 18706783

Description

EGLN1/PHD2 Polyclonal specifically detects EGLN1/PHD2 in Human, Mouse, Rat, Primate samples. It is validated for Western Blot, Simple Western, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Knockdown Validated.

Specifications

• Antigen: EGLN1/PHD2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: Tris-Citrate/Phosphate (pH 7.0 - 8.0) with 0.09% Sodium Azide
• Gene Alias: C1orf12HPH2, EC 1.14.11, EC 1.14.11.-, ECYT3, egl nine homolog 1, egl nine homolog 1 (C. elegans), egl nine-like protein 1, HIF prolyl hydroxylase 2, HIF-PH2, HIFPH2 EGL nine (C.elegans) homolog 1, HIF-prolyl hydroxylase 2, HPH-2, Hypoxia-inducible factor prolyl hydroxylase 2, PHD2SM20, Prolyl hydroxylase domain-containing protein 2, SM-20, zinc finger MYND domain-containing protein 6, ZMYND6DKFZp761F179
• Host Species: Rabbit
• Molecular Weight of Antigen: 46 kDa
• Quantity: 0.1 mg
• Research Discipline: Cancer, Hypoxia
• Gene ID (Entrez): 54583
• Target Species: Human, Mouse, Rat, Primate
• Isotype: IgG

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunoassay, KnockDown
• Concentration: 1 mg/mL
• Dilution: Western Blot 1:500 - 1:2500, Simple Western 1:500, Flow Cytometry 3.0 mcg/mL, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence 1:50, Immunoprecipitation, Immunohistochemistry-Paraffin 1:10 - 1:500, Knockout Validated, Knockdown Validated
• Gene Accession No.: Q9GZT9
• Gene Symbols: EGLN1
• Immunogen: The epitope recognized by this antibody maps to a region between residues 1 and 50 of human PHD2/HIF Prolyl Hydroxylase 2 using the numbering given in entry NP_071334.1 (GeneID 54583).
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Human. This antibody does not appear to work in Mouse. Results have been mixed in Rat with success in western analysis and immunofluorescence on Rat endothelial cells and negative results with PC12 cells. Expected reactivity with Rabbit, Orangutan, Rhesus Monkey, Gorilla based on 100% sequence identity.
• Content And Storage: Store at 4C. Do not freeze.

For Research Use Only