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STEMCELL Technologies EasySep™ Human IgG+ Memory B Cell Isolation Kit

Product Code. 17177771
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17177771 For Cell Isolation
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Product Code. 17177771 Supplier STEMCELL Technologies Supplier No. 17868

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Description

Immunomagnetic cell isolation kit using particle release technology

  • The EasySep™ Human IgG+ Memory B Cell Isolation Kit isolates IgG+CD27+ B cells from fresh or previously frozen peripheral blood mononuclear cells (PBMCs) or leukapheresis samples
  • First, CD27+ cells are isolated by column-free immunomagnetic positive selection using EasySep™ Releasable RapidSpheres™
  • Then, bound magnetic particles are removed from the EasySep™-isolated CD27+ cells, and unwanted non-B cells, as well as IgM+, IgD+ and IgA+ cells are depleted using EasySep™ Dextran RapidSpheres™
  • The final isolated fraction contains IgG+CD27+ memory B cells, which readily secrete IgG in culture supernatants in the presence of appropriate stimuli

Specifications

Cell Type B Cells
For Use With (Application) Cell Isolation
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