Thermo Scientific™ DyLight™ Sulfhydryl-Reactive Fluorescent Dyes

Label antibodies or other proteins as probes for fluorescence imaging assays with the full spectral range of maleimide-activated DyLight Dyes.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 46618

DYLIGHT 680 MALEIMIDE 1MG

UNSPSC: 41116012

Code: PN

Additional Details:
Additional Details: Weight: 0.00010kg



Product Code. 10791775

Quantity Price
1 £ 192.0 / 1mg
Estimated Shipment date
from Supplier 02-01-2017
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  • Description and Specification
  • Documentation
  • Accessories and Spare Parts
Description and Specification

Specification

Description DyLight 680 Maleimide
Formulation Maleimide activated proprietary near-infrared fluorescent dye
Sufficient For 1 use to label a total of 8mg of IgG using typical conditions
Quantity 1mg

The Thermo Scientific™ DyLight™ Dyes have absorption spectra ranging from 350 to 770nm. These reagents fluoresce over a broad pH range, are more intense than Alexa Fluor™ or Cy™ Dyes in many applications and match the output wavelengths of common fluorescence instrumentation. Additionally, the water solubility of the DyLight Reagents allows a high fluor-to-protein ratio without precipitation during conjugation.
The sulfhydryl-reactive dyes contain maleimide groups that react predominantly with free –SH groups at pH 6.5-7.5, forming a stable thioether bond. At pH 7, the maleimide group is ~1000 times more reactive toward a free sulfhydryl than to an amine.1At pH values > 7.5, reactivity toward primary amines increases and hydrolysis of the maleimide group can occur.

Highlights:
  • Specific — maleimide-activated dye labels proteins and other molecules at reduced sulfhydryls (-SH)
  • Efficient labeling methods — well-characterized chemistry and optimized protocols provide for reliable, high-quality labeling
  • Optimized antibody labeling procedure —complete protocol for IgG reduction and labeling and calculating the labeling efficiency

Applications:
  • Antibody labeling for immunofluorescence applications, including immunocytochemistry (ICC), immunohistochemistry (IHC), Western blotting and ELISA assay
  • Target macromolecule labeling for in vitro and in vivo fluorescent detection strategies

Documentation

Technical documents

Accessories and Spare Parts