1 use to label a total of 6mg of IgG using typical conditions
Azide groups, which are the reactive targets of the phosphine-activated fluors, can be introduced into proteins or other cellular targets via azide reagents or through in vivo labeling with azide-derivatives of naturally occurring metabolic building blocks. Because neither phosphines nor azides are present in biological systems, they comprise chemoselective (mutually specific) ligation pairs for labeling and conjugation. Thus, when used in combination with azide labeling strategies, Phosphine-Activated DyLight Fluors enable directed fluorescent labeling to facilitate detection of protein interactions and post-translational modifications using fluorescence imaging technologies.
Soluble easily dissolves in water-miscible solvents (e.g., DMSO) for subsequent dilution in aqueous reaction mixtures with cell lysates and other biological samples
Compatible reaction chemistry occurs effectively in simple buffer conditions; requires no accessory reagents such as copper or reducing agents, and does not interfere with fluorescence applications
Chemoselective the phosphine reactive group is specific in biological samples for azide-tagged molecules, ensuring that fluorescent labeling is specific
High-performance fluorescence DyLight 488, DyLight 550 and DyLight 650 are intense, highly stable fluorophores for green, orange and red fluorescent detection