Invitrogen™ DTL Polyclonal Antibody

Rabbit Polyclonal Antibody

Product Code	Brand	Quantity
17188953	Invitrogen™ PA5111035	100 μL
17198953	Invitrogen™ PA5111036	100 μL

Description

Immunogen sequence: AGDLPLPSNT PTFSIKTSPA KARSPINRRG SVSSVSPKPP SSFKMSIRNW VTRTPSSSPP ITPPASETKI MSPRKALIPV SQKSSQA Highest antigen sequence indentity to the following orthologs: Mouse - 85%, Rat - 86%.

Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis.

Specifications

• Antigen: DTL
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Classification: Polyclonal
• Form: Liquid
• Isotype: IgG
• Target Species: Human
• Gene: DTL
• Gene Alias: 2810047L02Rik; 5730564G15Rik; CDT2; CDW1; DCAF2; DDB1 and CUL4 associated factor 2; DDB1- and CUL4-associated factor 2; denticleless E3 ubiquitin protein ligase homolog; denticleless E3 ubiquitin protein ligase homolog (Drosophila); denticleless homolog; denticleless homolog (Drosophila); denticleless protein homolog; DTL; L2dtl; lethal(2) denticleless protein homolog; Meth A RAMP; Meth A retinoic acid-regulated nuclear matrix-associated protein; RA regulated nuclear matrix associated protein; RAMP; RA-regulated nuclear matrix-associated protein; Retinoic acid-regulated nuclear matrix-associated protein; RGD1310439
• Gene Symbols: DTL
• Primary or Secondary: Primary
• Purification Method: Antigen affinity chromatography

• Concentration: 0.05 mg/mL
• Applications: Immunocytochemistry
• Conjugate: Unconjugated
• Host Species: Rabbit
• Regulatory Status: RUO
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: Q9NZJ0
• Gene ID (Entrez): 51514
• Immunogen: Recombinant Protein corresonding to Human DTL. Recombinant protein control fragment (Product #RP-105875).
• Product Type: Antibody