Thermo Scientific™ DreamTaq™ Hot Start Green DNA Polymerase

Thermo Scientific DreamTaq Hot Start DNA Polymerase is the product of choice when you need consistently robust amplification with minimal optimization steps

Brand:  DreamTaq EP1713

Product Code. 15649374

Includes: DreamTaq Hot Start DNA Polymerase; 10X DreamTaq Green Buffer 

Description

Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that ensures higher PCR specificity, sensitivity and yields compared to conventional hot start Taq DNA polymerases. DreamTaq Hot Start DNA Polymerase uses antibody based inhibition of DNA polymerase activity at ambient temperatures, thus preventing the amplification of non-specific products prior to the amplification step.

Better reaction outcomes:

• Higher yield of target amplicons from low template amounts.
• Increased reaction specificity due to reliable hot-start technology.
• Wider range of amplicon lengths - routine amplification of genomic DNA fragments up to 6kb.

Enhanced convenience:

• Room temperature reaction set-up.
• Minimized optimization of Mg2+ concentration and of primer annealing temperatures due to optimized reaction buffer.
• Direct loading of PCR products on a gel with DreamTaq Green buffer.

Amplicon size (complex DNA): 6 kb
Fidelity (vs. Taq): 1X

Recommended Storage

Store at -20C.

Specifications

• Content And Storage: Contains:
  • 5 x 100 μL DreamTaq Hot Start DNA Polymerase (5 U/μL)
  • 10 x 1.25 mL 10X DreamTaq Green Buffer (includes 20 mM MgCl₂)
  
  Store at -5 to -30°C.
• Polymerase: DreamTaq DNA Polymerase
• Product Line: DreamTaq™
• Shipping Condition: Wet or Dry Ice
• Overhang: 3'-A

• GC-Rich PCR Performance: Low
• Reaction Speed: Standard
• Quantity: 2500 U
• Hot Start: Built-In Hot Start
• Reaction Format: Separate Components