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Dendritic Cell Marker DCIR2 Monoclonal Antibody (33D1), eBioscience™, Invitrogen™

Rat Monoclonal Antibody

£40.98 - £191.00

Specifications

Antigen Dendritic Cell Marker DCIR2
Clone 33D1
Concentration 0.5 mg/mL
Applications Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen)
Classification Monoclonal
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Products 3
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15227557
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Affymetrix eBioscience
14-5884-82
100 μg
£73.75
100µg
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Estimated Shipment: 10-04-2024
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15217557
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Affymetrix eBioscience
14-5884-81
50 μg This item is currently unavailable or has been discontinued.
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15237557
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Affymetrix eBioscience
14-5884-85
500 μg This item is currently unavailable or has been discontinued.
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Description

Description

Description: The 33D1 monoclonal antibody reacts with a mouse dendritic cell (DC)-specific surface marker, DCIR2. The nature and biological activity of DCIR2 is unknown. DCIR2 has been reported on a variety of dendritic cell subpopulations from mouse thymus, spleen, lymph node, and Peyer's patch. Bone marrow dendritic cells require GM-CSF to express DCIR2, and this expression is downregulated in the presence of IL-4. DCIR2 has been detected in vivo in brain dendritic cells post infection with Toxoplasma gondii. Applications Reported: This 33D1 antibody has been reported for use in flow cytometric analysis, immunohistology staining of frozen tissue sections, and immunohistochemical staining. It has also been reported in complement-mediated cytotoxicity. Applications Tested: The 33D1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. For detection of purified 33D1 it is recommended not to use goat anti-rat due to its low affinity for 33D1. Rather, we recommend using biotin mouse anti-rat (cat. 13-4813).

The nature and biological activity of DCIR2 is unknown. DCIR2 has been reported on a variety of dendritic cell subpopulations from mouse thymus, spleen, lymph node, and Peyer's patch. Bone marrow dendritic cells require GM-CSF to express DCIR2, and this expression is downregulated in the presence of IL-4. DCIR2 has been detected in vivo in brain dendritic cells post infection with Toxoplasma gondii.
Specifications

Specifications

Dendritic Cell Marker DCIR2
0.5 mg/mL
Monoclonal
Liquid
RUO
474145
IgG2b κ
Affinity chromatography
Antibody
33D1
Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen)
Unconjugated
Rat
Mouse
Clec4a4
Primary
4° C
Clec4a4
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For Research Use Only.