Cytochrome b245 alpha Antibody (CS9) - BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
18294614	Novus Biologicals NBP1-40974-0.025MG	0.025 mg
18788863	Novus Biologicals NBP1-40974-0.1mg	0.1 mg

Description

Cytochrome b245 alpha Monoclonal specifically detects Cytochrome b245 alpha in Human samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry-Frozen.

Specifications

• Antigen: Cytochrome b245 alpha
• Concentration: 1.0 mg/mL
• Applications: ELISA, Immunohistochemistry (Frozen), Flow Cytometry, Immunoprecipitation, Western Blot, Immunohistochemistry
• Conjugate: Unconjugated
• Host Species: Mouse
• Target Species: Human
• Gene Accession No.: P13498
• Gene ID (Entrez): 1535
• Immunogen: Cytochrome B245 solubilised in octyl-beta-gluycopyranoside
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

• Clone: CS9
• Dilution: Western Blot 1:100-1:2000, Flow Cytometry 1:10-1:100, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Formulation: PBS (pH 7.4) with 0.09% Sodium Azide
• Gene Alias: cytochrome b light chain, Cytochrome b(558) alpha chain, cytochrome b(558) alpha-subunit, cytochrome b, alpha polypeptide, cytochrome b-245 light chain, cytochrome b-245, alpha polypeptide, Cytochrome b558 subunit alpha, flavocytochrome b-558 alpha polypeptide, Neutrophil cytochrome b 22 kDa polypeptide, p22 phagocyte B-cytochrome, p22phox, p22-phox, Superoxide-generating NADPH oxidase light chain subunit
• Gene Symbols: CYBA
• Isotype: IgG1
• Purification Method: Protein G purified
• Test Specificity: This antibody recognizes the light chain of cytochrome B245, also known as p22phox, a subunit of the nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase). NADPH oxidase is a membrane-bound enzyme complex in neutrophils that produces the superoxide anion (O2-) by transferring single electrons from NADPH inside the cell across the membrane and coupling them to molecular oxygen. Superoxide is used for the destruction of pathogens. Individuals with defects in NADPH oxidase suffer from chronic granulomatous disease, characterized by severe recurrent infections.

For Research Use Only