CXCL10 (IP-10) Monoclonal Antibody (4NY8UN), Alexa Fluor™ 647, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen 51-9744-41

Product Code. 16656394

Description

Description: This 4NY8UN monoclonal antibody reacts with human CXCL10. CXCL10, also known as Interferon Inducible Protein-10 (IP-10), is a member of the CXC family of chemokines. It is secreted by monocytes, epithelial cells, and endothelial cells in response to IFN gamma or other pro-inflammatory cytokines and stimuli. CXCL10 signaling is mediated by the G protein-coupled receptor CXCR3, which is expressed on activated T cells and plays an important role in directing the migration of T cells, especially during Th1 responses. In addition to its chemotactic functions, CXCL10 is a potent inhibitor of angiogenesis and tumor growth and modulates expression of adhesion molecules. Elevated levels of CXCL10 are observed in response to allergic inflammation, various infections, and Alzheimer′s disease. Applications Reported: This 4NY8UN antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This 4NY8UN antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to 'Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins' located at Flow Protocols. This may be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

IP-10 (interferon-gamma induced protein 10 kD; in mouse, also called cytokine responsive gene 2, Crg-2, or mob-1) is a highly inducible, primary response gene that belongs to the CXC chemokine superfamily. It was first cloned in 1985. The biological functions of IP-10 are still unclear. Like Mig, IP-10 has no activity on neutrophils. Its functions include stimulation of monocytes, natural killer and T-cell migration, regulation of T-cell and bone marrow progenitor maturation, modulation of adhesion molecule expression as well as inhibition of angiogenesis. Mig and IP-10 share the same receptor, CXCR3.

Specifications

• Antigen: CXCL10 (IP-10)
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P02778
• Gene Symbols: CXCL10
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 3627
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: 4NY8UN
• Conjugate: Alexa Fluor 647
• Gene: CXCL10
• Gene Alias: 10 kDa interferon gamma-induced protein; BOS_6808; C Cmotif chemokine; C X C motif chemokine; C7; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 10; chemokine C-X-C motif ligand 10; chemokine CXC-like protein; Crg2; crg-2; CXC; CXC motif chemokine; C-X-C motif chemokine 10; C-X-C motif chemokine 10-like protein; C-X-C motif chemokine ligand 10; CXCL; Cxcl10; CXCL10(1-73); gamma interferon inducible protein 10; gamma IP10; Gamma-IP10; gIP-10; H-IP-10; Ifi10; Inp10; interferon activated gene 10; Interferon-gamma induced protein CRG-2; interferon-gamma-induced protein CRG-2; interferon-gamma-inducible protein 10; interferon-gamma-inducible protein-10; interferon-inducible cytokine IP-10; Interferon-inducible protein 10; IP10; IP-10; M-IP-10; Mob1; mob-1; protein 10 from interferon (gamma)-induced cell line; Protein Mob-1; SCYB10; small inducible cytokine B subfamily (Cys-X-Cys) member 10; small inducible cytokine B subfamily (Cys-X-Cys), member 10; small inducible cytokine subfamily B (Cys-X-Cys), member 10; Small-inducible cytokine B10
• Host Species: Mouse
• Quantity: 25 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2b κ