Invitrogen™ CXCL1 (GRO alpha) Monoclonal Antibody (KTYFLF), eFluor™ 660, eBioscience™

Mouse Monoclonal Antibody

Brand:  Invitrogen™ 50-7515-42

Product Code. 15530047

Description

Description: This KTYFLF monoclonal antibody reacts with human CXCL1 and has no cross-reactivity to human CXCL2 or CXCL3. CXCL1, or GRO (growth-related oncogene) alpha, is a pro-inflammatory CXC chemokine first identified by its constitutive overexpression in some tumors. It is closely related to CXCL2 (GRO beta) and CXCL3 (GRO gamma), with which it shares 90% and 86% sequence homology, respectively. These proteins, along with IL-8 or CXCL8, were later found to be critical for neutrophil mobilization and degranulation, as well as vascular permeabilization and angiogenesis. Signaling occurs through the G protein-coupled receptor CXCR2, which is shared with GRO beta and gamma. CXCR2 can also be activated by IL-8, although some studies suggest that the majority of IL-8 activity on neutrophils is mediated by CXCR1. CXCL1 is secreted by monocytes, epithelial cells, and fibroblasts in response to pro-inflammatory stimuli such as LPS, IL-1 beta, and TNF alpha. Overexpression is observed in many malignant tumors, where it contributes to tumor vascularization and metastasis. Applications Reported: This KTYFLF antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This KTYFLF antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of peripheral blood monocytes. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that wi...

CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.

Specifications

• Antigen: CXCL1 (GRO alpha)
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P09341
• Gene Symbols: Cxcl1
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 2919
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: KTYFLF
• Conjugate: eFluor 660
• Gene: Cxcl1
• Gene Alias: alpha-chemokine; C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 1; chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha); Cinc1; CINC-1; CXC; CXC motif chemokine; C-X-C motif chemokine 1; C-X-C motif chemokine ligand 1; CXCL; CXCL1; CXCL-1; Cytokine-induced neutrophil chemoattractant 1; fibroblast secretory protein; Fsp; Gro; GRO a; GRO α; Gro1; GRO1 oncogene; GRO1 oncogene (melanoma growth stimulating activity, alpha); GRO1 oncogene (melanoma growth-stimulating activity); GROa; GROalpha; GRO-alpha(1-73); GRO-alpha(4-73); GRO-alpha(5-73); GRO-alpha(6-73); Growth-regulated alpha protein; GROα; Hematopoietic synergistic factor; HSF; KC; KC(5-72); KC/GR)-alpha; KC/GRO-alpha; KC-T; melanoma growth stimulating activity, alpha; Melanoma growth stimulatory activity; melanoma growth stimulatory activity alpha; MGSA; MGSA alpha; MGSA-a; N51; NAP3; NAP-3; Neutrophil-activating protein 3; platelet-derived growth factor-inducible protein KC; SCYB1; secretory protein N51
• Host Species: Mouse
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ