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Invitrogen™ CellTrace™ Oregon Green™ 488 Carboxylic Acid Diacetate, Succinimidyl Ester (Carboxy-DFFDA, SE), cell permeant, mixed isomers
Description
Like CFDA SE, CellTrace™ Oregon Green™ 488 (carboxy-DFFDA SE) should be a useful tool for following proliferating cells. This Oregon Green™ 488 probe passively diffuses into cells, where it is colorless and nonfluorescent until its acetate groups are removed by intracellular esterases to yield a highly fluorescent, amine-reactive dye. Upon reaction with intracellular amines, the probe forms Oregon Green™ 488 conjugates that are well-retained by cells. Unlike fluorescein conjugates, Oregon Green™ 488 conjugates exhibit bright, photostable green fluorescence that is independent of pH at typical cellular pH values (pH-6-8).
Specifications
Specifications
| Content And Storage | Contains 20 vials of Oregon Green™ 488 carboxylic acid diacetate, SE (50 μg/vial). Store in freezer (-5 to -30°C) and protect from light. |
| Detection Method | Fluorescence |
| For Use With (Application) | Cell Viability and Proliferation |
| For Use With (Equipment) | Fluorescence Microscope, Flow Cytometer |
| Product Type | Carboxy-DFFDA SE |
| Dye Type | Oregon Green™ 488 |
| Emission | 488 |
| Form | Solid |
| Product Line | CellTrace, Oregon Green |
| Quantity | 20 x 50 μg |
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For Research Use Only. Not for use in diagnostic procedures.
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