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Invitrogen™ CellTrace™ CFSE Cell Proliferation Kit, for flow cytometry

CellTrace™ CFSE Cell Proliferation Kit, for flow cytometry

£73.25 - £188.00

Specifications

Detection Method Fluorescent
Excitation Wavelength Range 492nm
For Use With (Equipment) For flow cytometer
Quantity 1 Kit(s)
Storage Requirements –5 to –30°C
View More Specs

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Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
10013162 View Documents Invitrogen™
C34554
1 Kit(s) £188.00
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15598431 View Documents Invitrogen™
C34570
1 Kit(s) £73.25
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Description

Description

CellTrace™ CFSE Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry.

  • Superior performance—bright, single-peak staining enables visualization of multiple generations
  • Long-term signal stability—well-retained in cells for several days post stain
  • Non-toxic—staining does not adversely effect cell health
  • Simple, robust staining protocol

    View a selection guide for all CellTrace™ Cell Proliferation Kits for flow cytometry.

    Superior fluorescent staining
    Successful proliferation analysis by dye dilution (see figure below) requires an extremely bright dye to distinguish fluorescently labeled cells from auto-fluorescence after several cell divisions. The intense fluorescent staining provided by CellTrace™ CFSE dye enables the visualization of eight or more generations of proliferating cells before the signal is overwhelmed by intrinsic cellular auto-fluorescence. Consistent, homogeneous staining results in very little fluorescence variation between cells in a population, so distinct generations can be seen without any requirement for complex modeling software.

    Long-term signal retention
    Unlike stains that label the lipid membrane of cells, CellTrace™ CFSE dye easily crosses the plasma membrane and covalently binds inside cells where the stable, well-retained fluorescent dye is designed to provide a consistent signal, even after several days in a cell culture environment.

    Non-toxic
    CellTrace™ CFSE dye binds covalently to all free amines on the surface and inside of cells and shows little cytotoxicity, with minimal observed effect on the proliferative ability or biology of cells. Researchers have used CFSE SE labeling to show that transplantable hematopoietic cells proliferate in vitro in response to stimulation by a growth factor cocktail.

    Simple, robust staining protocol
    The CellTrace™ CFSE Cell Proliferation Kit contains convenient single-use vials of dry dye to permit small-scale experiments without preparing excess quantities of dye. A stock solution is prepared by dissolving the contents of a vial in anhydrous DMSO prior to use. To stain 1 mL of cells in protein-free medium, 1μL of this stock solution is typically used. Cells should be stained for 20 minutes at room temperature with gentle agitation. A brief wash with complete medium will then quench any dye remaining in solution.
  • Specifications

    Specifications

    Fluorescent
    For flow cytometer
    –5 to –30°C
    492nm
    1 Kit(s)
    CellTrace™ Cell Proliferation Kit

    For Research Use Only. Not for use in diagnostic procedures.