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Thermo Scientific™ CellSensor™ NFAT bla HEK 293T Cell Line

Product Code. 10667363
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Quantity:
1 Vial
Unit Size:
Each
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Product Code. 10667363

Brand: Thermo Scientific™ K1538

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Description

The CellSensor™ NFAT-bla HEK 293T cell line contains a beta-lactamase reporter gene under the control of the Nuclear Factor of Activated T Cells (NFAT) response element stably integrated into HEK 293T cells.

The CellSensor™ NFAT-bla HEK 293T cell line contains a beta-lactamase reporter gene under the control of the Nuclear Factor of Activated T Cells (NFAT) response element stably integrated into HEK 293T cells. Fluorescence activated cell sorting (FACS) was used to isolate clones responsive to stimulation of the NFAT pathway by PMA (Phorbol 12-Myristate 13-Acetate) and Thapsagargin (Thaps). The cell line was validated for DMSO tolerance, cell number, stimulation time, substrate loading conditions, and EC50 concentration of Thaps at fixed concentration of PMA. The CellSensor™ NFAT-bla HEK 293T cell line responds to agonist treatment as expected from the literature and can be adapted for high-throughput screening (including coupling of receptors to the NFAT pathway) for agonists or antagonists with compound libraries. Candidate drugs can also be tested for dose response against this cell line. Academic and non-profit customers, please inquire for special pricing.

TRUSTED_SUSTAINABILITY

Specifications

Assay Entry Cell-based beta lactamase reporter gene
Cell State Dividing Cells
Content And Storage CellSensor™ Cell Lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
Description CellSensor™ NFAT bla HEK 293T Cell Line, 1Vial, ∽ 2,000,000 cells/vial at 2,000,000 cells/mL Quantity of Cells, NFAT bla HEK 293T Cell Line, Fluorescent Detection Method, CellSensor™ Druggable Target, Adherent Growth Properties, Liquid Nitrogen Storage
Detection Method Fluorescent
For Use With (Application) Pharma and biopharma, drug discovery and development, target and lead identification and validation, kinase biology, kinase pathway analysis, pathway biology, cellular pathway analysis assays, cellSensor™ cellular pathway assays
Includes beta-lactamase reporter gene under the control of the nuclear factor of activated T cells (NFAT) response element stably integrated into HEK 293T cells
Product Line CellSensor™
Quantity 1 Vial
Sample Type NFAT-bla HEK 293T
Target Entry PKC, PKC and calcium pathway
Type Cell Line
System Type CellSensor™
Druggable Target Signaling Pathway
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For Research Use Only. Not for use in diagnostic procedures.

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