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Thermo Scientific™ CellSensor™ ISRE-bla Jurkat Cell Line

Product Code. 10489993
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Quantity:
1 Vial
Unit Size:
Each
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Product Code. 10489993

Brand: Thermo Scientific™ K1656

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Description

The CellSensor™ ISRE-bla Jurkat cell line contains a beta-lactamase reporter gene under control of the interferon-stimulated response element (ISRE) stably integrated into Jurkat cells.

The CellSensor™ ISRE-bla Jurkat cell line contains a beta-lactamase reporter gene under control of the interferon-stimulated response element (ISRE) stably integrated into Jurkat cells. To construct this cell line, the ISRE-bla construct was transduced into Jurkat cells by lentivirus. Flow cytometry was used to isolate cells responsive to IFNα. This cell line was validated for DMSO tolerance, cell number, stimulation time, substrate loading time, as well as ' and EC50 concentrations of IFNα. The CellSensor™ ISRE-bla Jurkat cell line is responsive to Interferon alpha (IFN-alpha) and Interferon beta and can be used to probe the type I interferon-induced JAK-STAT signaling pathways. This cell line can be adapted for high-throughput screening of agonist or antagonist compound libraries. Candidate drugs can also be tested for dose response against this cell line. ISRE-bla Jurkat cells were treated with agonist IFN-alpha; over the indicated concentration range in a 384-well format. Cells were incubated for 5 hours with agonist and 0.5% DMSO and then combined with LiveBLAzer™-FRET B/G Substrate (CCF4-AM) for 2 hours. Fluorescence emission values at 460 nm and 530 nm were obtained using a standard fluorescence plate reader, and the 460/530 ratios were plotted against the concentration of the agonist. Academic and non-profit customers, please inquire for special pricing.

TRUSTED_SUSTAINABILITY

Specifications

Assay Entry Cell-based beta lactamase reporter gene
Cell State Dividing Cells
Content And Storage CellSensor™ Cell Lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
Description CellSensor™ ISRE-bla Jurkat Cell Line, 1Vial, ∽ 5,000,000 cells/vial at 5,000,000 cells/mL Quantity of Cells, ISRE-bla Jurkat Cell Line, Fluorescent Detection Method, CellSensor™ Druggable Target, Suspension Growth Properties, Liquid Nitrogen Storage
Detection Method Fluorescent
For Use With (Application) Pharma and biopharma, drug discovery and development, target and lead identification and validation, pathway biology, cellular pathway analysis assays, cellsensor™ cellular pathway assays
Includes beta-lactamase reporter gene under control of the interferon-stimulated response element (ISRE) stably integrated into Jurkat cells
Product Line CellSensor™
Quantity 1 Vial
Sample Type ISRE-bla Jurkat
Target Entry JAK/STAT (Type I IFN) pathway, TYK2, JAK1
Type Cell Line
System Type CellSensor™
Druggable Target Signaling Pathway
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For Research Use Only. Not for use in diagnostic procedures.

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