Invitrogen™ eBioscience™ Cell Stimulation Cocktail (plus protein transport inhibitors) (500X)

Can be used for induction and subsequent intracellular detection of cytokines and other secreted proteins in both in vitro-cultured and ex vivo cells

Brand:  Invitrogen™ 00-4975-93

Product Code. 15516286

Description

Cocktail of phorbol 12-myristate 13-acetate (PMA), ionomycin, brefeldin A and monensin. Treatment with PMA and ionomycin is sufficient to induce activation of many cell types to produce cytokines. Brefeldin A and Monensin result in the accumulation of secreted proteins in the endoplasmic reticulum and Golgi apparatus

• A cocktail of phorbol 12-myristate 13-acetate (PMA), ionomycin, brefeldin A and monensin
• Treatment with PMA and ionomycin is sufficient to induce activation of many cell types to produce cytokines
• Brefeldin A and Monensin result in the accumulation of secreted proteins in the endoplasmic reticulum and Golgi apparatus
• Thus, it can be used for induction and subsequent intracellular detection of cytokines and other secreted proteins in both in vitro-cultured and ex vivo cells

Specifications

• Product Type: Cell Stimulation Cocktail
• Quantity: 100 μL
• For Use With (Application): Flow Cytometry, Functional Assays, Cell Culture
• Product Line: eBioscience™

For Research Use Only. Not for use in diagnostic procedures.