Invitrogen™ CD56 (NCAM) Recombinant Mouse Monoclonal Antibody (CMSSB), Alexa Fluor™ Plus 488, Invitrogen™

Mouse Recombinant Monoclonal Antibody

Brand:  Invitrogen™ 740024MP48820UG

Product Code. 30131983

Description

Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro. Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Applications Tested: This CMSSB antibody has been tested by IHC and flow cytometric analysis of NK-92cells. This may be used for IHC at 10 μg/mL and for FC at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 494 nm; Emission: 519 nm; Laser: Blue Laser Filtration: 0.2 μm post-manufacturing filtered.

CD56, also known as neural cell adhesion molecule (NCAM), is a highly glycosylated transmembrane glycoprotein of the immunoglobulin family. It plays a crucial role in cell adhesion, migration, axonal growth, pathfinding, and synaptic plasticity. CD56 is ubiquitously expressed in the nervous system in isoforms ranging from 120-180 kDa and is involved in homotypic adhesion of neural cells. It mediates interactions by binding extracellular matrix components such as laminin and integrins, with polysialic modification reducing CD56-mediated adhesion. In the hematopoietic system, CD56 is expressed on natural killer (NK) cells and a subset of T cells known as NKT cells. It is also found on most neuroectodermal-derived cell lines, tissues, and neoplasms, including retinoblastoma, medulloblastoma, astrocytomas, and neuroblastoma. CD56 serves as a widely used neuroendocrine marker with high sensitivity for neuroendocrine tumors and ovarian granulosa cell tumors. Diseases associated with CD56 dysfunction include rabies and blastic plasmacytoid dendritic cell neoplasms, highlighting its importance in both neural and immune system functions.

Specifications

• Antigen: CD56 (NCAM)
• Classification: Recombinant Monoclonal
• Concentration: 1.0 mg/mL
• Formulation: Proprietary buffer with 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone; pH 6.8
• Gene Accession No.: P13591
• Gene Symbols: Ncam1
• Purification Method: Protein A/G
• Regulatory Status: RUO
• Gene ID (Entrez): 4684
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry, Immunocytochemistry
• Clone: CMSSB
• Conjugate: Alexa Fluor Plus 488
• Gene: Ncam1
• Gene Alias: adhesion molecule; antigen recognized by monoclonal antibody 5.1H11; Cd56; CD-56; CD56 120 kDa GPI-linked isoform; CD56 140 kDa isoform; CD56 140 kDa VASE isoform; E NCAM; E-NCAM; MSK39; N CAM1; NCAM; N-CAM; Ncam1; N-CAM-1; NCAM-1; NCAMC; NCAM-C; neural cell adhesion molecule; Neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM; sCD56; sNCAM; soluble CD56; soluble NCAM
• Host Species: Mouse
• Quantity: 20 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ