CD41a Monoclonal Antibody (HIP8), Super Bright™ 436, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
16391154	eBioscience 62-0419-41	25 Tests
16301164	Invitrogen™ 62-0419-42	100 Tests

Description

Description: The HIP8 monoclonal antibody reacts with the human CD41 molecule, the integrin alphaIIb also known as platelet GPIIb. CD41 non-covalently associates with integrin beta3 (GPIIIa, CD61) and is expressed by megakaryocytes and platelets. The CD41/CD61 complex is a receptor for fibronectin, fibrinogen, von Willebrand factor, vitronectin and thrombospondin and mediates platelets aggregation. HIP8 blocks platelet aggregation. Applications Reported: This HIP8 antibody has been reported for use in flow cytometric analysis. Applications Tested: This HIP8 antibody has been pre-diluted and tested by flow cytometric analysis of mouse splenocytes. This may be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product No. SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

CD41 (platelet glycoprotein IIb, ITGA2B) is composed of two subunits -120 kDa a, alpha and 23 kDa b, beta- that interact with CD61 in the presence of calcium to form a functional adhesive protein receptor. CD41 is also involved in blood coagulation by mediating platelet aggregation. Upon blood vessel damage, CD41 binds to a variety of proteins including von Willebrand factor, fibrinogen, fibronectin and vitronectin. CD41 is mainly expressed on megakaryocyte-platelet lineage, but generally belongs to the antigens that are expressed during the early stages of hematopoietic differentiation. Diseases associated with CD41 dysfunction include Glansmann Thrombasthenia, and Platelet type-16 bleeding disorder.

Specifications

• Antigen: CD41a
• Concentration: 5 μL/Test
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: P08514
• Isotype: IgG1 κ
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!

• Clone: HIP8
• Applications: Flow Cytometry
• Conjugate: Super Bright 436
• Host Species: Mouse
• Target Species: Human
• Gene ID (Entrez): 3674
• Primary or Secondary: Primary
• Product Type: Antibody