Invitrogen™ CD277 Monoclonal Antibody (eBioBT3.1 (20.1, BT3.1)), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
17190612	Invitrogen™ 14-2779-37	2 mg
17100622	Invitrogen™ 14-2779-95	10 mg

Description

The eBioBT3.1 MAb recognizes BT3.1, BT3.2 and BT3.3. The BT family of proteins is a subgroup of the Ig superfamily (IgSF). BT is a glycoprotein that forms a major component of the milk fat globule molecule. In addition to BT, there are six other genes that have been discovered which have been sub-divided into the BT2 (BT2.1, BT2.2, and BT2.3) and BT3 (BT3.1, BT3.2, and BT3.3) families. BT3.1, BT3.2, and BT3.3 share 95% mRNA identity. BT3 molecules are constitutively expressed on the cell surface of antigen-presenting cells, and the IgV-like domain of BT3.1 is sufficient for interaction with its counter-receptor. BT3.1 is expressed by T cells, B cells and CD14+ cells. Applications Reported: This eBioBT3.1 (20.1, BT3.1) antibody has been reported for use in flow cytometric analysis, and immunoprecipitation. Applications Tested: This eBioBT3.1 (20.1, BT3.1) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

The butyrophilin (BTN) genes are a group of major histocompatibility complex (MHC)-associated genes that encode type I membrane proteins with 2 extracellular immunoglobulin (Ig) domains and an intracellular B30.2 (PRYSPRY) domain. Three subfamilies of human BTN genes are located in the MHC class I region: the single-copy BTN1A1 gene and the BTN2 and BTN3 genes, which have undergone tandem duplication, resulting in 3 copies of each.

Specifications

• Antigen: CD277
• Concentration: 0.5 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: BT3.1; BTF5; BTN3.1; BTN3A1; butyrophilin; Butyrophilin subfamily 3 member A1; butyrophilin, subfamily 3, member A1; CD277; dJ45P21.3 (butyrophilin, subfamily 3, member A1)
• Gene Symbols: BTN3A1
• Primary or Secondary: Primary
• Content And Storage: 4°C
• Gene: BTN3A1

• Clone: eBioBT3.1 (20.1, BT3.1)
• Applications: Flow Cytometry, Immunoprecipitation
• Conjugate: Unconjugated
• Host Species: Mouse
• Target Species: Human
• Gene Accession No.: O00481
• Gene ID (Entrez): 11119
• Isotype: IgG1
• Purification Method: Affinity Chromatography
• Product Type: Antibody