CD25 Rat, anti-Mouse, PerCP-Cyanine5.5, Clone: PC61.5, STEMCELL Technologies

Rat Monoclonal Antibody

Brand:  Stemcell Technologies 60009PS.1

Product Code. 17198401

Description

• The PC61.5 antibody reacts with mouse CD25 (low-affinity interleukin‐2 receptor α chain or IL-2Rα), which is an ∼55 kDa type 1 transmembrane glycoprotein expressed on T and B cell progenitors, activated (but not resting) T and B cells, regulatory T (Treg) cells, dendritic cells, and activated monocytes and macrophages
• Expression of CD25, together with CD4 and FOXP3, is considered a phenotypic signature for Treg cells
• CD25 per se has low affinity for its IL-2 ligand but associates with CD122 (IL-2Rβ) and CD132 (IL-2Rγ) to form the high-affinity IL-2R receptor
• CD25 is necessary for receptor clustering and induction of signaling pathways involved in the activation and proliferation of lymphocytes
• Binding of the PC61.5 antibody inhibits binding of IL-2 to the low- and high-affinity receptors, which may result in inhibition of IL-2-dependent proliferation
• The epitope recognized by PC61.5 is distinct from the IL-2 binding site and from the epitopes recognized by antibodies 7D4 and 3C7

Specifications

• Antigen: CD25
• Classification: Monoclonal
• Conjugate: PerCP-Cyanine5.5
• Quantity: 25 μg
• Target Species: Mouse
• Cell Type: T Cells, T Cells, Regulatory

• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Blocking Assay
• Clone: PC61.5
• Host Species: Rat
• Primary or Secondary: Primary
• Isotype: IgG1 λ