CD202b (TIE2) Monoclonal Antibody (TEK4), eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Brand:  Invitrogen 14-5987-81

Product Code. 15277667

Description

Description: The TEK4 monoclonal antibody reacts with mouse Tie-2, also known as CD202. A member of the tyrosine kinase receptor family, Tie-2 is expressed on endothelial and a subset of hematopoietic cells and is believed to play a role in both angiogenesis and hematopoiesis during development of the mouse embryo. In fetal liver and adult bone marrow, Tie-2 is expressed by a subpopulation of hematopoietic progenitor cells characterized as Lineage markers^-, c-Kit^+, Sca1^+ cells. Long-term multilineage repopulating cells were detected in Tie-2^+, Lineage^-, c-Kit^+, Sca1^+ cells but not in Tie-2^-, Lineage^-, c-Kit^+, Sca1^+ cells. Applications Reported: This TEK4 antibody has been reported for use in flow cytometric analysis, and immunohistology staining of frozen tissue sections. Applications Tested: The TEK4 antibody has been tested by blocking of staining with fluorochrome conjugated TEK4 on mouse bone marrow cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

Filtration: 0.2 μm post-manufacturing filtered. The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells in mice, rats, and humans. This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. The ligand for the receptor is angiopoietin-1. Defects in TEK are associated with inherited venous malformations; the TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase.

Specifications

• Antigen: CD202b (TIE2)
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q02858
• Gene Symbols: Tek
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 21687
• Content And Storage: 4° C
• Form: Liquid

• Applications: Flow Cytometry, Immunohistochemistry (Frozen)
• Clone: TEK4
• Conjugate: Unconjugated
• Gene: Tek
• Gene Alias: CD202B, TIE-2, TIE2, VMCM, VMCM1, angiopoietin-1 receptor, endothelial tyrosine kinase, hTIE2, p140 TEK, soluble TIE2 variant 1, soluble TIE2 variant 2, tunica interna endothelial cell kinase, tyrosine kinase with Ig and EGF homology domains-2, tyrosine-protein kinase receptor TEK, tyrosine-protei
• Host Species: Rat
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG1 κ

For Research Use Only.