Invitrogen™ CD200 Monoclonal Antibody (OX90), eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Product Code	Brand	Quantity
17120662	Invitrogen™ 14-5200-37	2 mg
17130662	Affymetrix eBioscience 14-5200-95	10 mg

Description

Description: The monoclonal antibody OX90.1 recognizes mouse CD200 also known as OX2. CD200 is a member of the Ig superfamily with 2 Ig domains, a transmembrane and cytoplasmic domain. CD200 is expressed on resting and activated B cells, a subset of resting and activated T cells, keratinocytes, peripheral and central nerve cells, follicular dendritic cells and ovarian cells. The interaction with CD200R results in macrophage activation (IL-6 production), inhibition of mast cell degranulation along with reduced TNF alpha and IL-13 secretion and overall attenuation of the activation status of lymphocytes. Applications Reported: This OX90 antibody has been reported for use in flow cytometric analysis, immunohistology staining of frozen tissue sections, and immunohistology staining of paraffin embedded tissue sections. Applications Tested: This OX90 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

CD200 is a type-1 membrane glycoprotein that contains two immunoglobulin domains, and belongs to the immunoglobulin superfamily. CD200 is a broadly distributed cell surface glycoprotein that interacts with a receptor on myeloid cells (CD200R) and is implicated in locally preventing macrophage activation. Distant, but recognizable, homologues of CD200 have been identified in many herpesviruses and poxviruses. Studies of the related genes in mouse and rat suggest that CD200 may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues. Multiple alternatively spliced transcript variants that encode different isoforms of CD200 have been found. Diseases associated with CD200 dysfunction include Mantle Cell Lymphoma and Primary Hypertrophic Osteoarthropathy.

Specifications

• Antigen: CD200
• Concentration: 0.5 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene ID (Entrez): 17470
• Primary or Secondary: Primary
• Content And Storage: 4°C
• Gene: Cd200

• Clone: OX90
• Applications: Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
• Conjugate: Unconjugated
• Host Species: Rat
• Target Species: Mouse
• Gene Accession No.: O54901
• Isotype: IgG2a κ
• Purification Method: Affinity chromatography
• Product Type: Antibody