CD200 Monoclonal Antibody (OX90), eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-5200-95

Product Code. 17130662

Description

Description: The monoclonal antibody OX90.1 recognizes mouse CD200 also known as OX2. CD200 is a member of the Ig superfamily with 2 Ig domains, a transmembrane and cytoplasmic domain. CD200 is expressed on resting and activated B cells, a subset of resting and activated T cells, keratinocytes, peripheral and central nerve cells, follicular dendritic cells and ovarian cells. The interaction with CD200R results in macrophage activation (IL-6 production), inhibition of mast cell degranulation along with reduced TNF alpha and IL-13 secretion and overall attenuation of the activation status of lymphocytes. Applications Reported: This OX90 antibody has been reported for use in flow cytometric analysis, immunohistology staining of frozen tissue sections, and immunohistology staining of paraffin embedded tissue sections. Applications Tested: This OX90 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

CD200 Monoclonal antibody specifically detects CD200 in Mouse samples. It is validated for Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)

Specifications

• Antigen: CD200
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: O54901
• Host Species: Rat
• Quantity: 10 mg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a κ

• Applications: Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
• Clone: OX90
• Conjugate: Unconjugated
• Gene: Cd200
• Gene Alias: antigen identified by monoclonal antibody MRC OX-2; CD200; CD200 antigen; CD200 molecule; cell surface protein (thymocyte antigen identified by monoclonal antibody MRC-OX2); Cspmo2; MOX1; MOX2; MRC; MRC OX-2 antigen; MRCOX2; My033; OX2; OX-2; OX-2 membrane glycoprotein
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 17470
• Content And Storage: 4° C
• Form: Liquid